【摘要】：中介體復(fù)合物在真核生物蛋白編碼基因和非編碼RNA的轉(zhuǎn)錄中非常重要。為了分析大豆中介體亞基的序列特征和基因表達(dá)模式,應(yīng)用BLASTp獲取與擬南芥中介體亞基同源的蛋白序列,用Clustal Omega、MAFFT、MEGA等方法進(jìn)行多序列聯(lián)配和進(jìn)化分析,同時(shí)應(yīng)用Me V構(gòu)建了基因的表達(dá)量熱圖。結(jié)果表明:共獲得118個(gè)基因位點(diǎn)(Locus),186個(gè)轉(zhuǎn)錄本,在大豆20條染色體上均有分布。Med8的N端具有TBP結(jié)構(gòu)域,Med31的C端具有脯氨酸富集區(qū)和假定的核定位信號(hào),Med16的C端有C2-C2型鋅指結(jié)構(gòu)域(Zinc-finger motif)。栽培大豆與野生大豆的同源蛋白親緣關(guān)系最近,進(jìn)化分析符合物種進(jìn)化規(guī)律。大豆不同中介體亞基基因的表達(dá)量差異很大,但同一基因在不同組織中的表達(dá)差異較小,僅有3個(gè)基因在根瘤中特異表達(dá),1個(gè)基因在豆莢中特異表達(dá)。脫水處理后5個(gè)中介體亞基基因表達(dá)上調(diào)明顯,Na Cl處理1和6 h后分別有2和3個(gè)中介體亞基基因表達(dá)顯著上調(diào)。這為進(jìn)一步分析中介體亞基在大豆發(fā)育和逆境脅迫中的分子功能奠定基礎(chǔ)。
[Abstract]:Intermediate complexes are very important in the transcription of eukaryotic protein-encoded genes and non-coding RNA. In order to analyze the sequence characteristics and gene expression patterns of soybean intermediary subunits, BLASTp was used to obtain protein sequences homologous to Arabidopsis intermediary subunits, and multiple sequence association and evolutionary analysis were carried out by Clustal Omega,MAFFT,MEGA and other methods. At the same time, the heat map of gene expression was constructed by using Me V. The results showed that a total of 118 (Locus), transcripts were obtained and distributed on 20 chromosomes of soybean. The N-terminal of Med8 had TBP domain, and the C-terminal of Med31 had proline-rich region and assumed nuclear localization signal. The C terminal of Med16 has C2C2 zinc finger domain (Zinc-finger motif). The phylogenetic relationship of homologous proteins between cultivated soybean and wild soybean was recent, and the evolutionary analysis was in accordance with the evolution law of species. The expression of subunits of different mediators in soybean varied greatly, but the expression of the same gene in different tissues was small. Only 3 genes were specifically expressed in root nodules and 1 gene was specifically expressed in pods. The gene expression of 5 mediators subunits was up-regulated after, Na Cl treatment for 1 h and 6 h, respectively, and the gene expressions of 2 and 3 intermediary subunits were up-regulated after dehydration treatment. This provides a basis for further analysis of the molecular functions of intermediary subunits in soybean development and stress.
【作者單位】： 河南省農(nóng)業(yè)科學(xué)院經(jīng)濟(jì)作物研究所;河南農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31371652) 國(guó)家高技術(shù)研究發(fā)展計(jì)劃“863計(jì)劃”(2012AA101106)
【分類號(hào)】：S565.1

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