【摘要】：本研究針對木薯淀粉品質(zhì)改良問題,提出通過調(diào)控質(zhì)體分裂元件,影響庫器官中淀粉體分裂來改變淀粉粒形態(tài)、大小及充實程度的新思路。主要研究成果如下：完成ftsZ2基因家族全長片段在木薯的克隆,利用生物信息學(xué)預(yù)測功能及分析進(jìn)化關(guān)系;利用FtsZ蛋白異常表達(dá)能嚴(yán)重影響大腸桿菌細(xì)胞分裂的特性,對內(nèi)共生起源的木薯質(zhì)體分裂基因MeftsZ2基因家族的功能進(jìn)行初步鑒定;以我國木薯主栽品種SC8遺傳轉(zhuǎn)化體系,構(gòu)建組成型和塊根特異型啟動子驅(qū)動的MeftsZ2-1基因的過量表達(dá)載體轉(zhuǎn)化木薯,轉(zhuǎn)化MeftsZ2-1基因的相關(guān)載體已得到經(jīng)過分子檢測的幾十個轉(zhuǎn)基因株系,并成功在田間種植,初步觀察到苗期MeftsZ2-1轉(zhuǎn)基因植株的葉柄和塊根表皮顏色較野生型明顯變紅,表現(xiàn)出與質(zhì)體發(fā)育相應(yīng)表型的改變：此外還對多個品種淀粉粒度、淀粉體發(fā)育及淀粉粒發(fā)生過程進(jìn)行了觀察,為利用該途徑改良木薯淀粉品質(zhì)的新思路提供理論依據(jù)。
[Abstract]:In order to improve the quality of cassava starch, a new idea was put forward to change the shape, size and enrichment of starch grains by regulating the plastid division elements and affecting the amyloplast division in reservoir organs. The main research results are as follows: complete the cloning of ftsZ2 gene family in cassava, using bioinformatics to predict the function and analyze the evolutionary relationship; The abnormal expression of FtsZ protein can seriously affect the characteristics of cell division in E. coli, and the function of the MeftsZ2 gene family of cassava plastids derived from endosymbiosis was preliminarily identified. Based on the SC8 genetic transformation system of cassava main cultivar in China, the overexpression vector of MeftsZ2-1 gene driven by constitutive and tuber special type promoter was constructed to transform cassava into cassava. Dozens of transgenic lines have been obtained by molecular detection and successfully planted in the field. The color of petiole and root epidermis of MeftsZ2-1 transgenic plants at seedling stage is obviously red than that of wild type. The phenotypic changes corresponding to plastid development were also observed. In addition, starch granularity, amyloid development and starch granulation process of many varieties were observed, which provided theoretical basis for improving cassava starch quality by this way.
【學(xué)位授予單位】：海南大學(xué)
【學(xué)位級別】：博士后
【學(xué)位授予年份】：2016
【分類號】：S533

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1 閔義;木薯質(zhì)體分裂相關(guān)基因ftsZ2家族的克隆及功能分析[D];海南大學(xué);2016年

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本文編號：2428854