【摘要】：以白蘇子(Perilla frutescens)花青素糖基轉(zhuǎn)移酶基因?yàn)樘结?通過電子克隆和RT-PCR的方法從金魚草(Antirrhinum majus L.)葉片中克隆到花青素糖基轉(zhuǎn)移酶基因(Am GT1)的全長c DNA,并對其表達(dá)特性進(jìn)行分析。結(jié)果表明:Am GT1全長892 bp,編碼277個氨基酸;進(jìn)化分析表明Am GT1的氨基酸序列與白蘇子的同源性最高為79%,與其他花青素糖基轉(zhuǎn)移酶蛋白的同源性在42%~62%之間,表明Am GT1是從金魚草中克隆的新的花青素糖基轉(zhuǎn)移酶基因。實(shí)時定量RT-PCR分析表明:該基因在金魚草葉片中的表達(dá)量最高,根中的表達(dá)量最低;該基因雖然在紅色、粉色、黃色、白色花朵中均有表達(dá),但在紅色花朵中的表達(dá)量最高,且存在一個從緊蕾期到松蕾期的躍變。
[Abstract]:(Perilla frutescens) anthocyanidin glycosyltransferase was used as a probe, and (Antirrhinum majus L.) was obtained by electronic cloning and RT-PCR. The full-length c DNA, of anthocyanin glycosyltransferase gene (Am GT1) was cloned from leaves and its expression characteristics were analyzed. The results showed that the total length of Am GT1 was 892 bp, encoding 277 amino acids. Evolutionary analysis showed that the amino acid sequence of Am GT1 had the highest homology with Perilla perilla, and the homology with other anthocyanin glycosyltransferase proteins was between 42% and 62%. The results indicate that Am GT1 is a new anthocyanin glycosyltransferase gene cloned from Goldfish. Real-time quantitative RT-PCR analysis showed that the expression of the gene was the highest in the leaves and the lowest in the roots. Although the gene was expressed in red, pink, yellow and white flowers, it had the highest expression in red flowers, and there was a jump from tight bud stage to pine bud stage.
【作者單位】： 漳州城市職業(yè)學(xué)院園林園藝系;貴州省煙草科學(xué)研究院/煙草行業(yè)分子遺傳重點(diǎn)實(shí)驗(yàn)室;
【基金】：中國煙草總公司貴州省公司項(xiàng)目(No.201501)
【分類號】：S681.9

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