【摘要】：為研究黃顙魚(Pelteobagrus fulvidraco)胰島素樣生長因子結(jié)合蛋白1基因(IGFBP1)的特征,闡明IGFBP1基因在不同性別黃顙魚組織中的表達(dá)特征和IGFBP1蛋白在雌、雄黃顙魚肌肉中的表達(dá)差異,克隆IGFBP1基因的c DNA全長,并進(jìn)行實(shí)時熒光定量PCR檢測和Western Blot分析。結(jié)果表明:黃顙魚IGFBP1基因c DNA全長為1 259 bp,其開放閱讀框長度為714 bp,編碼237個氨基酸,3'端和5'端非翻譯區(qū)分別為407 bp和138 bp;黃顙魚IGFBP1基因核苷酸序列與鲇形目斑點(diǎn)叉尾洶的相似度(89%)較高,與鰱、鯉、鯽、草魚和團(tuán)頭魴等的差異較大;肝臟是黃顙魚IGFBP1基因m RNA表達(dá)最豐富的組織,IGFBP1基因在雄性個體心臟、腎臟、肌肉和腸組織中的表達(dá)量顯著高于雌性的(P0.05);IGFBP1蛋白在雌、雄黃顙魚肌肉組織中都有表達(dá),且在雄性個體中的表達(dá)量高于在雌性個體中的表達(dá)量。
[Abstract]:In order to study the characteristics of (Pelteobagrus fulvidraco) insulin-like growth factor binding protein-1 (IGFBP1) gene in Pelteobagrus fulvidraco, the expression of IGFBP1 gene in different sex tissues and the difference of IGFBP1 protein expression in female and realgar Pelteobagrus fulvidraco muscle were studied. The full length of c DNA of IGFBP1 gene was cloned, and real-time fluorescence quantitative PCR detection and Western Blot analysis were performed. The results showed that the length of c DNA of IGFBP1 gene of Pelteobagrus fulvidraco was 1 259 bp, the length of open reading frame was 714 bp, encoding 237 amino acids, the untranslated region of 3 'end and 5' end were 407 bp and 138 bp;, respectively. The nucleotide sequence of IGFBP1 gene of Pelteobagrus fulvidraco had a high similarity (89%) with the spotted forked tail of Silurus catfish, and was different from that of silver carp, common carp, crucian carp, grass carp and bream. The expression of IGFBP1 gene in the heart, kidney, muscle and intestine of male individuals was significantly higher than that in female (P0.05). The expression of IGFBP1 protein was higher in male than in female.
【作者單位】： 中國水產(chǎn)科學(xué)研究院長江水產(chǎn)研究所;淡水水產(chǎn)健康養(yǎng)殖湖北省協(xié)同創(chuàng)新中心;
【基金】：“十二·五”農(nóng)村領(lǐng)域國家科技計劃項(xiàng)目(2011AA100401) 水產(chǎn)種質(zhì)資源平臺項(xiàng)目(2016DKA30470)
【分類號】：S917.4

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