【摘要】：目的探討pokemon基因表達與胃癌細胞藥敏性及耐藥基因的關(guān)系。方法取60例胃癌組織和癌旁組織石蠟標本,采用免疫組化技術(shù)檢測組織中pokemon蛋白及耐藥相關(guān)蛋白P-糖蛋白(P-gp)、多藥耐藥相關(guān)蛋白1(MRP1)、肺耐藥蛋白(LRP)、生存素(survivin)的表達,并分析pokemon蛋白與其他4種蛋白的關(guān)系。采用磺酰羅丹明B(SRB)蛋白染色法檢測5-氟尿嘧啶(5-FU)、順鉑(DDP)、奧沙利鉑(L-OHP)對胃癌細胞株SGC7901、胃癌耐藥細胞株SGC7901/ADR、胃上皮細胞株GES-1的抑制作用;qPCR及蛋白質(zhì)印跡法檢測上述3種細胞株中pokemon和耐藥基因的表達。合成針對pokemon的siRNA并轉(zhuǎn)染SGC7901/ADR細胞,檢測轉(zhuǎn)染前后SGC7901/ADR細胞的化療藥敏性及耐藥基因的變化。結(jié)果胃癌組織中pokemon、P-gp、MRP1、LRP、survivin蛋白的陽性表達率均高于癌旁組織(P0.05);pokemon蛋白表達與P-gp、survivin蛋白表達間存在正相關(guān)關(guān)系(r=0.385 2,P=0.002;r=0.342 4,P=0.007)。Pokemon、P-gp、MRP1、survivin蛋白在SGC7901/ADR細胞株中的表達高于細胞株SGC7901及GES-1;SGC7901/ADR細胞株的耐藥性最強,SGC7901次之,GES-1最弱(P0.01)。Pokemon-siRNA轉(zhuǎn)染SGC7901/ADR細胞后細胞中pokemon mRNA的表達明顯受到抑制;轉(zhuǎn)染后化療藥物對細胞的抑制能力增強,P-gp、survivin表達減弱(P0.05)。結(jié)論 Pokemon與P-gp、survivin通過相互調(diào)控而促進胃癌耐藥性的形成。Pokemon可能成為胃癌靶向治療的候選基因。
[Abstract]:Objective to investigate the relationship between pokemon gene expression and drug sensitivity and drug resistance genes in gastric cancer cells. Methods pokemon protein, P- glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1) and lung resistance protein (LRP), were detected by immunohistochemical technique in 60 cases of gastric cancer and paracancerous tissues. The expression of survivin (survivin) and the relationship between pokemon protein and other four proteins were analyzed. Detection of 5-fluorouracil (5-FU) and cisplatin (DDP), oxaliplatin (L-OHP) in the treatment of gastric cancer cell line SGC7901, by using sulfonyl rhodamine B (SRB) protein staining to detect the effect of 5-fluorouracil (5-FU) and cisplatin (DDP), oxaliplatin (L-OHP) on SGC7901, cell line SGC7901/ADR, Inhibition of gastric epithelial cell line GES-1; QPCR and Western blot were used to detect the expression of pokemon and drug resistance genes. SiRNA targeting pokemon was synthesized and transfected into SGC7901/ADR cells. The chemosensitivity and drug resistance genes of SGC7901/ADR cells were detected before and after transfection. Results the positive expression rate of pokemon,P-gp,MRP1,LRP,survivin protein in gastric cancer tissues was higher than that in paracancerous tissues (P0.05). There was a positive correlation between the expression of); pokemon protein and the expression of P-gpP pokemon,P-gp,MRP1,LRP,survivin protein (r = 0.385 2, P = 0.002). The expression of Pokemon,P-gp,MRP1,survivin protein in SGC7901/ADR cell line was higher than that in SGC7901 and GES-1; cell line. The drug resistance of SGC7901/ADR cell line was the strongest, SGC7901 was the second, and GES-1 was the weakest (P0.01). The expression of pokemon mRNA was significantly inhibited after Pokemon-siRNA transfection into SGC7901/ADR cells. After transfection, the inhibitory effect of chemotherapeutic drugs on cells was enhanced, and the expression of P-gpP survivin was decreased (P0.05). Conclusion Pokemon and P-gpP survivin promote the formation of drug resistance in gastric cancer through mutual regulation. Pokemon may be a candidate gene for targeted therapy of gastric cancer.
【作者單位】： 河北醫(yī)科大學(xué)第四醫(yī)院外三科;
【基金】：國家自然科學(xué)基金(81372580) Supported by National Natural Science Foundation of China(81372580) 河北省自然科學(xué)基金(H2016206173) Natural Science Foundation of Hebei Province(H2016206173) 河北省衛(wèi)生和計劃生育委員會重點科技研究計劃項目(20150352) Key Program of Science and Technology Research of Health and Family Planning Commission of Hebei(20150352)
【分類號】：R735.2

【相似文獻】

相關(guān)期刊論文 前9條

1 趙智宏;王勝發(fā);禹亮;王巨;常浩;張健;;Pokemon基因在非小細胞肺癌細胞系中的表達[J];哈爾濱醫(yī)科大學(xué)學(xué)報;2008年03期

2 高偉;隋軍;李曉江;任艷鑫;;Pokemon在腫瘤中的研究進展[J];中國醫(yī)學(xué)文摘(腫瘤學(xué));2007年04期

3 張紀妍;隋麗華;陳杰;賈長茹;;Pokemon在卵巢癌中的表達及臨床意義[J];臨床腫瘤學(xué)雜志;2014年02期

4 劉鑫遠;禹亮;張健;王磊;;Pokemon及其在腫瘤中的研究進展[J];現(xiàn)代生物醫(yī)學(xué)進展;2014年03期

5 徐海;崔明;;Pokemon蛋白與腫瘤[J];醫(yī)學(xué)綜述;2007年20期

6 徐湘江;高志遠;徐娟;張全樂;李衛(wèi)民;祁月英;龔汶;;肝癌TACE治療前后Pokemon表達變化及臨床意義[J];胃腸病學(xué)和肝病學(xué)雜志;2013年07期

7 張雨;余昌俊;宋海屏;;Pokemon蛋白在胃黏膜不典型增生和胃癌中的表達[J];安徽醫(yī)科大學(xué)學(xué)報;2007年06期

8 侯列軍;鄭軍華;耿江;顧聞宇;王波;;原癌基因pokemon的mRNA在腎癌中的表達及其意義[J];中國現(xiàn)代醫(yī)生;2011年27期

9 白玉賢;劉磊;隋紅;魏孝禮;苑珩珩;;miRNA干擾Pokemon基因?qū)Υ竽c癌細胞增殖的影響及機制[J];臨床腫瘤學(xué)雜志;2011年11期

相關(guān)會議論文 前1條

1 蔣宇揚;;Pokemon介導(dǎo)的腫瘤信號轉(zhuǎn)導(dǎo)通路的研究[A];第六屆全國化學(xué)生物學(xué)學(xué)術(shù)會議論文摘要集[C];2009年

相關(guān)碩士學(xué)位論文 前3條

1 馬雯倩;胃癌中Pokemon、PCNA、Bcl-2表達與其臨床生物學(xué)行為關(guān)系的研究[D];河北醫(yī)科大學(xué);2016年

2 王俊杰;Pokemon在腎癌中的表達及臨床意義[D];大連醫(yī)科大學(xué);2012年

3 宋玉健;Pokemon在腎癌中的表達及其臨床意義[D];大連醫(yī)科大學(xué);2013年

，

本文編號：2423382