【摘要】：本研究克隆了達(dá)氏鱘(Acipenser dabryanus)生長轉(zhuǎn)化因子gdf11(growth differentiation factor 11)基因cDNA。達(dá)氏鱘gdf11基因cDNA序列全長1 298 bp(不包括Poly A),開放閱讀框?yàn)? 191 bp,編碼396個(gè)氨基酸,5非編碼區(qū)長19 bp;3非編碼區(qū)長88 bp。通過Signal P軟件預(yù)測含有N端21aa的信號肽。組織表達(dá)特征分析結(jié)果顯示,gdf11在7種組織中均有表達(dá),在眼和鰓中的表達(dá)量較高;不同水流條件下,鰓和心臟gdf11的表達(dá)量有顯著性差異,靜水中鰓gdf11表達(dá)量是流水(流速27.0±3.0 cm/s)的4.70倍,而心臟中的表達(dá)量是流水的2.72倍。這暗示了gdf11可能在呼吸代謝中發(fā)揮功能。
[Abstract]:In this study, the gene cDNA. of (Acipenser dabryanus) growth transformation factor gdf11 (growth differentiation factor 11 was cloned. The cDNA sequence of gdf11 gene of Sturgeon da Sturgeon is 1 298 bp (excluding Poly A), open reading frame: 1 191 bp, encodes 396 amino acids, 5 non-coding region is 19 bp;3 length 88 bp.) The signal peptide containing N-terminal 21aa was predicted by Signal P software. The results of tissue expression analysis showed that gdf11 was expressed in all 7 tissues, and higher in eyes and gills. Under different water flow conditions, the expression of gdf11 in Gill and heart was significantly different. The expression of gdf11 in Gill was 4.70 times of that in flowing water (27.0 鹵3.0 cm/s) and 2.72 times in heart. This suggests that gdf11 may function in respiratory metabolism.
【作者單位】： 中國水產(chǎn)科學(xué)研究院長江水產(chǎn)研究所農(nóng)業(yè)部淡水生物多樣性保護(hù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：公益性農(nóng)業(yè)行業(yè)專項(xiàng)(200903048) 國家自然基金青年基金項(xiàng)目(31202003) 中國長江三峽集團(tuán)公司科技環(huán)保項(xiàng)目
【分類號】：S917.4
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本文編號：2422002