【摘要】：目的探討藥物蟾蜍靈(Bufalin)對T系白血病jurkat細胞的作用。方法體外培養(yǎng)人T系白血病的細胞株(Jurkat),以濃度0、5、25、100nmol/l藥物Bufalin作用jurkat細胞株,細胞計數(shù)試劑盒(cck-8)測定jurkat抑制率,膜聯(lián)蛋白(annexi)V/碘化丙啶(PI)雙染后,流式細胞儀用于測量jurkat細胞的凋亡率,蛋白質(zhì)印跡(Western Blotting)檢測與細胞凋亡相關(guān)蛋白c-myc,p57kip2的變化及Na,K-ATPase的變化.結(jié)果cck-8檢測結(jié)果顯示:jurkat經(jīng)蟾蜍靈處理24小時后,jurkat的細胞用蟾蜍靈藥物處理后,對其增殖有明顯受抑作用;流式細胞儀用來檢測結(jié)果表明:蟾蜍靈可以使jurkat細胞發(fā)生凋亡;蛋白質(zhì)印跡檢測結(jié)果顯示:不同濃度蟾蜍靈藥物處理jurkat細胞24小時后與細胞凋亡相關(guān)蛋白。結(jié)論蟾蜍靈可以使jurkat細胞株發(fā)生凋亡,下調(diào)的c-myc,上調(diào)的p57kip2可能參與這一過程。
[Abstract]:Objective to investigate the effect of bufalin (Bufalin) on T-lineage leukemia jurkat cells. Methods (Jurkat), a human T lineage leukemia cell line cultured in vitro, was incubated with Bufalin at a concentration of 25100nmol / l. The inhibition rate of jurkat was measured by cell count kit (cck-8), and the double staining of (annexi) V / (PI) was performed. Flow cytometry was used to measure the apoptosis rate of jurkat cells. Western blot (Western Blotting) was used to detect the changes of apoptosis related protein c-mycu p57kip2 and Na,K-ATPase. Results the results of cck-8 test showed that the proliferation of jurkat cells treated with bufalin for 24 hours was significantly inhibited after treated with bufalin. The results of flow cytometry showed that bufalin could induce apoptosis in jurkat cells, and Western blot analysis showed that different concentrations of bufalin were associated with apoptosis in jurkat cells after 24 hours of treatment with different concentrations of bufalin. Conclusion Bufalin can induce apoptosis of jurkat cell line and down-regulate c-myc, and up-regulated p57kip2 may participate in this process.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R733.7

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1 熊安秀;王敏;金潤銘;白燕;林雯;;哇巴因誘導(dǎo)Jurkat細胞凋亡與胱冬酶-3及bcl-2基因家族的關(guān)系[J];中國實驗血液學(xué)雜志;2006年05期

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