【摘要】：目的探討表皮生長因子受體(EGFR)基因表達(dá)對胃癌分期的影響及誘導(dǎo)胃癌細(xì)胞增殖凋亡的機(jī)制。方法應(yīng)用免疫組化法檢測100例胃癌組織中EGFR基因的表達(dá),分析其與臨床病理特征的關(guān)系。培養(yǎng)胃癌細(xì)胞株SGC-7901,實(shí)驗(yàn)分為空白對照組、空載體組、EGFR-siRNA組,3組細(xì)胞轉(zhuǎn)染12、24、48、72 h后,CCK8檢測EGFR基因?qū)?xì)胞增殖的影響;流式細(xì)胞儀檢測EGFR基因?qū)?xì)胞凋亡的影響;Western印跡檢測凋亡相關(guān)蛋白的表達(dá)。結(jié)果 100例胃癌組織中,EGFR表達(dá)陽性41例,對照組無陽性表達(dá),差異有統(tǒng)計(jì)學(xué)意義(P0.01);胃癌分化程度、浸潤程度、淋巴轉(zhuǎn)移中EGFR的表達(dá)差異顯著(P0.05),TNM分期與EGFR的表達(dá)差異極顯著(P0.01);隨著時(shí)間的延長,3組細(xì)胞的增殖率均上升,EGFR-siRNA組增殖較緩慢,24 h的增殖率與空白對照組和空載體組比較顯著降低(P0.05),48 h和72 h的增殖率與空白對照組和空載體組比較顯著降低(P0.01);3組細(xì)胞轉(zhuǎn)染48 h后,EGFR-siRNA組細(xì)胞的凋亡率顯著低于空白對照組和空載體組(P0.01);空白對照組和空載體組細(xì)胞中Bcl-2、Bax、酶切Caspase3 3個(gè)蛋白表達(dá)均無顯著差異(P0.05),EGFR-siRNA組細(xì)胞中Bax、酶切Caspase3蛋白表達(dá)顯著高于空白對照組和空載體組,Bcl-2蛋白表達(dá)顯著低于空白對照組和空載體組(P0.01)。結(jié)論 EGFR基因的表達(dá)參與胃癌生長、侵襲、轉(zhuǎn)移和分化過程,下調(diào)EGFR基因的表達(dá)能抑制胃癌細(xì)胞株SGC-7901增殖,促進(jìn)細(xì)胞凋亡,凋亡可能與Bcl-2、Bax、Caspase3的調(diào)控有關(guān)。
[Abstract]:Objective to investigate the effect of epidermal growth factor receptor (EGFR) gene expression on gastric cancer staging and the mechanism of inducing proliferation and apoptosis of gastric cancer cells. Methods Immunohistochemical method was used to detect the expression of EGFR gene in 100 cases of gastric cancer and its relationship with clinicopathological features was analyzed. Gastric cancer cell line SGC-7901, was divided into three groups: blank control group, empty vector group, EGFR-siRNA group, and 3 groups. After transfection of 12 ~ 24N ~ 48 ~ 72 h, CCK8 was used to detect the effect of EGFR gene on cell proliferation, flow cytometry was used to detect the effect of EGFR gene on cell apoptosis. The expression of apoptosis-related protein was detected by Western blot. Results among the 100 cases of gastric cancer, 41 cases were positive for EGFR, while no positive expression was found in the control group (P0.01). The expression of EGFR in lymph node metastasis and differentiation degree of gastric cancer was significantly different (P0.05 between), TNM stage and EGFR expression (P0.01). With the prolongation of time, the proliferation rate of the three groups increased, and the proliferation rate of EGFR-siRNA group was slower than that of blank control group and empty carrier group (P0.05), and the proliferation rate of 24 h group was significantly lower than that of blank control group and empty carrier group (P0.05). The proliferation rate of 48 h and 72 h was significantly lower than that of blank control group and empty carrier group (P0.01). The apoptosis rate of EGFR-siRNA group was significantly lower than that of blank control group and empty vector group 48 h after transfection (P0.01). There was no significant difference between blank control group and empty vector group in the expression of Bcl-2,Bax, enzyme digested Caspase3 protein (P0.05). The expression of Bax, enzyme digested Caspase3 protein in EGFR-siRNA group was significantly higher than that in blank control group and empty vector group. The expression of Bcl-2 protein was significantly lower than that of blank control group and empty vector group (P0.01). Conclusion the expression of EGFR gene is involved in the process of growth, invasion, metastasis and differentiation of gastric cancer. Down-regulating the expression of EGFR gene can inhibit the proliferation of gastric cancer cell line SGC-7901 and promote apoptosis, which may be related to the regulation of Bcl-2,Bax,Caspase3.
【作者單位】： 重慶市急救醫(yī)療中心病理科;西南醫(yī)科大學(xué)附屬第一醫(yī)院病理科;
【基金】：瀘州醫(yī)學(xué)院自然科學(xué)基金青年基金項(xiàng)目(No.2013ZRQN031)
【分類號】：R735.2

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