【摘要】：以葡萄無核品種無核白和有核品種黑比諾為材料,克隆葡萄CONSTANS基因家族的1個成員并將其命名為VvCOL5基因,分析基因結構,進行染色體定位和系統(tǒng)發(fā)育樹的構建,檢測VvCOL5基因在無核和有核葡萄胚珠(幼種子)發(fā)育過程的表達變化。結果表明:VvCOL5基因cDNA長度為1 374bp,包含有2個外顯子和1個內含子結構,并含有2個B-box結構域和1個CCT結構域,屬于CO家族的第一類,在GenBank登錄號為KX077245。在受精后胚珠(幼種子)發(fā)育過程中,VvCOL5基因在無核白與黑比諾中呈現出了相反的表達模式。其無核白受精后10~30d其表達緩慢上調,30~35d維持在較高水平,35~40d緩慢下降,后在40~45d又快速上調到較高水平;而在黑比諾中10~20d下降,在20~35d之間相對平穩(wěn),35~40d緩慢上升,最后40~45d時又下降到較低水平。無核白中VvCOL5基因在各時期的表達量均高于黑比諾,可見VvCOL5基因在無核和有核品種中表達差異較大,其與葡萄種子發(fā)育有一定關系。
[Abstract]:A member of grape CONSTANS gene family was cloned from seedless grape varieties denuclearized and Pinot Noir, named VvCOL5 gene, gene structure was analyzed, chromosome mapping and phylogenetic tree construction were carried out. To detect the expression of VvCOL5 gene during the development of seedless and nucleated grape ovule (young seeds). The results show that the length of cDNA of VvCOL5 gene is 1 374 BP, including 2 exons and 1 intron structure, 2 B-box domains and 1 CCT domain, which belong to the first class of CO family, and the GenBank accession number is KX077245.. During the development of ovules (young seeds) after fertilization, the VvCOL5 gene expressed in denuclearized and Pinot Noir. The expression increased slowly at 10 ~ 30 days after fertilization, maintained at a higher level at 30 ~ 35 days, decreased slowly at 35 ~ 40 days, and then increased to a higher level at 40 ~ 45 days after fertilization. In Pinot Noir, it decreased at 10 ~ 20 days, remained stable at 20 ~ 35 days, increased slowly at 35 ~ 40 days, and then decreased to a lower level at 40 ~ 45 days. The expression of VvCOL5 gene in denuclearized white was higher than that in Pinot noir at every stage. It was found that the expression of VvCOL5 gene in seedless and nucleated varieties was different and had a certain relationship with grape seed development.
【作者單位】： 西北農林科技大學園藝學院旱區(qū)作物逆境生物學國家重點實驗室農業(yè)部西北地區(qū)園藝作物生物學與種質創(chuàng)制重點實驗室;
【基金】：國家自然科學基金(31372023) 國家現代農業(yè)產業(yè)技術體系建設專項(CARS-30-yz-7) 楊凌示范區(qū)農業(yè)科技示范推廣項目(2015-TS-10)
【分類號】：S663.1
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本文編號：2405844