【摘要】：為了探究補(bǔ)體C9在尼羅羅非魚(Oreochromis niloticus)免疫中發(fā)揮的作用,本研究克隆并分析了尼羅羅非魚補(bǔ)體C9基因(OnC9)。結(jié)果顯示:OnC9的cDNA序列全長2 502 bp,包含1 761 bp的開放閱讀框(ORF),編碼586個氨基酸。氨基酸序列同源性分析表明,OnC9與牙鲆(Paralichthys olivaceus)補(bǔ)體C9氨基酸相似性最高,達(dá)73.0%,與其他魚類補(bǔ)體C9的相似性介于49.3%~71.4%之間。實(shí)時熒光定量PCR檢測結(jié)果表明,OnC9基因在所檢測的各個組織或器官中表達(dá)水平有明顯差異,在肝臟中表達(dá)量最高,其次是腸道、后腎、皮膚、肌肉、鰓,在腦、脾臟、心臟、頭腎、胸腺和血液中表達(dá)量最低。在無乳鏈球菌(Streptococcus agalactiae)感染魚體后,肝臟、后腎等組織中OnC9表達(dá)量表現(xiàn)為在感染12 h、48 h(或36 h)、120 h先后出現(xiàn)三個峰值的波動表達(dá)的規(guī)律。說明OnC9在無乳鏈球菌感染尼羅羅非魚后的免疫應(yīng)答中發(fā)揮作用。
[Abstract]:In order to explore the role of complement C9 in Oreochromis niloticus (Oreochromis niloticus), this study cloned and analyzed the complement C9 gene of Oreochromis niloticus (OnC9). The results showed that the full length of the cDNA sequence of OnC9 was 2,502 bp, which contained an open reading frame (ORF) of 1,761 bp, encoding 586 amino acids. The homology of amino acid sequence analysis shows that the similarity of the amino acid of the complement C9 of the OnC9 and the Parallicthys olivaceus is the highest, the similarity of the amino acid sequence is 73.0%, and the similarity with the other fish complement C9 is between 49 and 3% to 71.4%. The real-time fluorescence quantitative PCR test showed that the expression level of the OnC9 gene in the detected tissues or organs was significantly different, the expression in the liver was the highest, followed by the intestinal tract, the back kidney, the skin, the muscle, the skin, the brain, the spleen, the heart, the head kidney, The amount of expression in the thymus and the blood is the lowest. The expression of the OnC9 in the tissues of the liver, the back kidney and the like after the infection of the fish body with Streptococcus agalactiae was shown as the rule of the fluctuation expression of the three peaks in the time of 12 h, 48 h (or 36 h) and 120 h. Note that the OnC9 plays a role in the immune response after the non-dairy streptococcus is infected with the Nile tilapia.
【作者單位】： 中國水產(chǎn)科學(xué)研究院珠江水產(chǎn)研究所農(nóng)業(yè)部熱帶亞熱帶水產(chǎn)資源利用與養(yǎng)殖重點(diǎn)實(shí)驗(yàn)室;上海海洋大學(xué)水產(chǎn)與生命學(xué)院;
【基金】：現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系專項(xiàng)資金(CARS-49) 國家自然科學(xué)基金(31502205) 廣東省自然科學(xué)基金(2014A030310337)
【分類號】：S917.4

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