【摘要】：促性腺激素釋放激素(GnRH)由GnRH編碼,是調(diào)節(jié)動(dòng)物繁殖活動(dòng)的核心神經(jīng)內(nèi)分泌物質(zhì)。布氏田鼠Lasiopodomys brandtii是我國(guó)內(nèi)蒙古東部草原區(qū)的害鼠之一,具有明顯季節(jié)性繁殖特征,但其繁殖調(diào)控機(jī)制仍未完全明確。本研究克隆了來(lái)自布氏田鼠下丘腦的GnRH cDNA序列,使用實(shí)時(shí)熒光定量技術(shù)檢測(cè)了不同組織、不同年齡階段GnRH mRNA水平。結(jié)果表明,克隆獲得GnRH cDNA序列497 bp,包含開(kāi)放閱讀框273 bp,編碼90個(gè)氨基酸和1個(gè)終止密碼子的GnRH前體。DNA序列比對(duì)和氨基酸序列同源性分析表明,布氏田鼠下丘腦GnRH基因?qū)儆冖裥?與橙腹田鼠Microtus ochrogaste GnRH1相似性最高。GnRH mRNA在下丘腦、垂體、睪丸、腎上腺、腸、膀胱均有表達(dá)。雄鼠血清睪酮水平和雌鼠血清雌二醇水平在出生后8周、36周、80周處于高水平,顯著高于4周齡鼠,但除80周齡鼠下丘腦GnRH表達(dá)量處于高水平,8周齡、36周齡鼠GnRH表達(dá)量與4周齡鼠差異均無(wú)統(tǒng)計(jì)學(xué)意義。推測(cè)4周齡鼠下丘腦GnRH受GnRH調(diào)節(jié)劑的中心抑制,而8周齡、36周齡鼠下丘腦GnRH受性類固醇介導(dǎo)的反饋抑制調(diào)控,當(dāng)年齡增加至80周齡,性類固醇介導(dǎo)的反饋抑制消失或者應(yīng)答時(shí)間延長(zhǎng)。本研究結(jié)果為探究布氏田鼠繁殖調(diào)控規(guī)律提供了更多基礎(chǔ)資料。
[Abstract]:Gonadotropin releasing hormone (GnRH), encoded by GnRH, is the core neuroendocrine substance regulating animal reproduction. Lasiopodomys brandtii is one of the pests in the eastern grassland of Inner Mongolia, which has obvious seasonal reproduction characteristics, but its reproductive regulation mechanism is not completely clear. In this study, GnRH cDNA sequences from the hypothalamus of voles brucelis were cloned, and GnRH mRNA levels in different tissues and ages were detected by real-time fluorescence quantitative technique. The results showed that the cloned GnRH cDNA sequence 497 bp, contained 90 amino acids and a stop codon GnRH precursor encoded by open reading frame 273 bp,. DNA sequence alignment and amino acid sequence homology analysis showed that, The GnRH gene in the hypothalamus of the vole belongs to type 鈪，

本文編號(hào)：2404906