【摘要】：油菜素類(lèi)固醇(Brassinosteroids,BRs)在調(diào)節(jié)植物生長(zhǎng)發(fā)育和提高植物對(duì)非生物脅迫的抗性中起著重要作用,CPD(Constitutive Photomorphogenesis and Dwarfism)基因編碼一種甾醇類(lèi)23a羥化酶,在BR的生物合成中發(fā)揮著重要作用.本研究克隆胡楊Pe CPD基因的c DNA序列,并利用農(nóng)桿菌介導(dǎo)法,將Pe CPD基因植物過(guò)表達(dá)載體p BI121-35S::Pe CPD轉(zhuǎn)化野生型煙草,篩選得到Pe CPD基因過(guò)表達(dá)的轉(zhuǎn)基因煙草植株,并利用Na Cl、高氮和甘露醇分別對(duì)轉(zhuǎn)基因和野生型煙草進(jìn)行脅迫處理,檢測(cè)轉(zhuǎn)基因煙草的耐受性和相關(guān)的生理生化指標(biāo).結(jié)果表明:(1)在正常條件下,轉(zhuǎn)基因煙草的鮮重是野生型煙草的1.47倍;(2)在Na Cl、高氮以及甘露醇的脅迫處理下,轉(zhuǎn)基因煙草植株的根長(zhǎng)、葉片葉綠素和類(lèi)胡蘿卜素的含量均顯著高于野生型植株,抗氧化酶SOD和POD活性以及滲透調(diào)節(jié)物質(zhì)脯氨酸、可溶性蛋白和可溶性糖的含量也同樣表現(xiàn)為轉(zhuǎn)基因煙草顯著高于野生型,而野生型植株的丙二醛(MDA)含量顯著高于轉(zhuǎn)基因植株.因此在煙草中異源過(guò)表達(dá)胡楊Pe CPD基因不但能夠增加煙草的生物量,而且能夠提高煙草對(duì)鹽、高氮和干旱脅迫的抵抗能力.
[Abstract]:Rapesin steroids (Brassinosteroids,BRs) play an important role in regulating plant growth and development and enhancing plant resistance to abiotic stress. The, CPD (Constitutive Photomorphogenesis and Dwarfism) gene encodes a sterol 23a hydroxylase. It plays an important role in the biosynthesis of BR. In this study, the c DNA sequence of Pe CPD gene of Populus euphratica was cloned and transformed into wild type tobacco by means of Agrobacterium tumefaciens mediated by Pe CPD gene plant overexpression vector p BI121-35S::Pe CPD. Transgenic tobacco plants with Pe CPD gene overexpression were screened. Na Cl, high nitrogen and mannitol were used to stress transgenic tobacco and wild type tobacco respectively to detect tolerance and related physiological and biochemical indexes of transgenic tobacco. The results showed that: (1) under normal conditions, the fresh weight of transgenic tobacco was 1.47 times of that of wild tobacco; (2) under the stress of Na Cl, high nitrogen and mannitol, the root length, chlorophyll and carotenoid content of transgenic tobacco plants were significantly higher than those of wild-type plants. The activities of antioxidant enzymes SOD and POD and the contents of proline, soluble protein and soluble sugar also showed that transgenic tobacco was significantly higher than wild-type tobacco. The malondialdehyde (MDA) content of wild type plants was significantly higher than that of transgenic plants. Therefore, heterologous expression of Populus euphratica Pe CPD gene in tobacco could not only increase the biomass of tobacco, but also enhance the resistance of tobacco to salt, high nitrogen and drought stress.
【作者單位】： 西南科技大學(xué)生命科學(xué)與工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31270660) 四川省青年科技基金項(xiàng)目(2014JQ0016) 西南科技大學(xué)博士啟動(dòng)資金和西南科技大學(xué)杰出青年基金項(xiàng)目資助~~
【分類(lèi)號(hào)】：Q943.2

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