【摘要】：本文以前期獲得的銀葉真蘚在干旱-復(fù)水過(guò)程中的轉(zhuǎn)錄組數(shù)據(jù)為基礎(chǔ),選擇了9個(gè)在干旱-復(fù)水條件下基因表達(dá)差異明顯的相關(guān)耐干響應(yīng)基因,并利用熒光定量PCR技術(shù)研究其在干旱-復(fù)水、ABA誘導(dǎo)脅迫及鹽脅迫下的表達(dá)模式。研究結(jié)果表明,RT-q PCR實(shí)驗(yàn)獲得的9個(gè)基因在干旱-復(fù)水下的表達(dá)趨勢(shì)與RNA-Seq基因表達(dá)譜中基因表達(dá)規(guī)律基本一致,因此較好地驗(yàn)證了RNA-Seq數(shù)據(jù)的可靠性。在干旱-復(fù)水過(guò)程中,9個(gè)基因均不同程度受到干旱和復(fù)水誘導(dǎo)表達(dá),但復(fù)水階段基因誘導(dǎo)表達(dá)更為強(qiáng)烈,且大多數(shù)基因表達(dá)量在復(fù)水2 h達(dá)到高峰,如ERF、Dehydrin(DHN)和HSF3基因等。在ABA和鹽脅迫處理下,9個(gè)耐干相關(guān)基因中有8個(gè)同時(shí)受ABA和鹽誘導(dǎo)上調(diào)表達(dá),其中ERF、DHN和LEA基因受ABA和鹽脅迫上調(diào)表達(dá)最為顯著。綜上,ERF、DHN和LEA等基因同時(shí)受干旱-復(fù)水,ABA和高鹽強(qiáng)烈誘導(dǎo)表達(dá),可作為將來(lái)重點(diǎn)研究的抗逆基因。
[Abstract]:Based on the transcriptional data obtained in the early stage of drought and rehydration, nine genes related to dry tolerance were selected, which showed significant difference in gene expression under the condition of drought and rehydration. Fluorescence quantitative PCR technique was used to study its expression pattern under drought rehydration ABA induced stress and salt stress. The results show that the expression trend of 9 genes obtained in RT-q PCR experiment is basically consistent with that in RNA-Seq gene expression profile. Therefore, the reliability of RNA-Seq data is well verified. During the course of drought and rehydration, the nine genes were induced to express by drought and rehydration to varying degrees, but the induced expression was stronger in the rehydration stage, and the expression of most genes reached its peak at 2 h after rehydration, such as ERF,. Dehydrin (DHN) and HSF3 genes. Under ABA and salt stress, 8 of the 9 dry-tolerant genes were up-regulated by ABA and salt-induced, and ERF,DHN and LEA genes were most significantly up-regulated by ABA and salt stress. In conclusion, genes such as ERF,DHN and LEA were strongly induced by drought rehydration, ABA and high salt, and could be used as stress resistant genes in the future.
【作者單位】： 中國(guó)科學(xué)院新疆生態(tài)與地理研究所中國(guó)科學(xué)院干旱區(qū)生物地理與生物資源重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院大學(xué);香港中文大學(xué)農(nóng)業(yè)生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：新疆維吾爾自治區(qū)自然科學(xué)基金面上項(xiàng)目(2015211A047)~~
【分類(lèi)號(hào)】：Q943.2

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