【摘要】：本研究根據(jù)扁桃生物信息學數(shù)據(jù)庫,采用PCR的方法從扁桃(Amygdalus communis L.)中克隆了一個CBF轉錄因子基因,命名AcCBF2,基因開放閱讀框(open reading frame,ORF)為717 bp,編碼238個氨基酸,預測其分子量26.59 kD,等電點為5.29。氨基酸多重序列比對顯示,AcCBF2基因編碼的氨基酸與其它植物冷脅迫相關的CBF氨基酸序列具有高度同源性,含有一個AP2功能結構域和2個特征基序;系統(tǒng)發(fā)育樹分析顯示,AcCBF2基因屬于DREB家族中的A-1亞族,熒光定量顯示,AcCBF2基因只對低溫和干旱脅迫有響應,對鹽脅迫和ABA(脫落酸)沒有明顯響應。在低溫脅迫下,表達量上調;而在干旱脅迫下,表達量先上調后下調。初步預測AcCBF2基因可能對扁桃非生物脅迫有重要的調控作用。
[Abstract]:In this study, according to the bioinformatics database of almond, (Amygdalus communis L.) of almond was obtained by using PCR method. An CBF transcription factor gene was cloned and named AcCBF2, open reading frame (open reading frame,ORF) encoding 238 amino acids for 717 bp,. The predicted molecular weight was 26.59 kD, isoelectric point (5.29). Amino acid multiple sequence alignment showed that the amino acids encoded by AcCBF2 gene had high homology with other plant CBF amino acids related to cold stress, and contained one AP2 functional domain and two characteristic motifs. Phylogenetic tree analysis showed that AcCBF2 gene belonged to A-1 subfamily of DREB family. Fluorescence quantitative analysis showed that AcCBF2 gene was only responsive to low temperature and drought stress, but not to salt stress and ABA (abscisic acid). Under low temperature stress, the expression level was up-regulated, while under drought stress, the expression was up-regulated and then down-regulated. It is preliminarily predicted that AcCBF2 gene may play an important role in the regulation of abiotic stress in almond.
【作者單位】： 新疆農(nóng)業(yè)大學;
【基金】：國家自然科學基金(31360473) 國家林業(yè)公益性行業(yè)科研專項(201304701-1) 新疆自治區(qū)園藝學重點學科基金共同資助
【分類號】：S662.9;Q943.2

【參考文獻】

相關期刊論文 前6條

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本文編號：2387860