發(fā)布時(shí)間：2018-12-15 03:16

【摘要】：為了探明弱光脅迫對(duì)葡萄葉片光合生理的影響,以4年生‘巨峰’葡萄為材料,在盛花后進(jìn)行20 d 70%遮光作為弱光處理,然后恢復(fù)至未遮光植株(對(duì)照)水平,測(cè)定果實(shí)品質(zhì)和葉片的光合參數(shù)、葉綠素?zé)晒�、葉綠素含量、葉片糖含量及組分、光合酶基因及蔗糖磷酸合成酶基因表達(dá)等。結(jié)果發(fā)現(xiàn),弱光處理導(dǎo)致成熟期葡萄果實(shí)可溶性固形物含量降低,可滴定酸含量升高,果皮花色苷含量減少,果實(shí)縱徑降低。隨著弱光時(shí)間延長(zhǎng),葉片葉綠素b含量增加,葉綠素a/b值下降,光合速率和光化學(xué)效率降低,葉片中可溶性糖含量降低,Rubisco大、小亞基,PRKase,SPS1-2,SPS3等基因表達(dá)受到抑制;光照恢復(fù)至對(duì)照水平10 d后,光合速率、光化學(xué)效率及可溶性糖含量略有上升,光合酶基因表達(dá)得到部分恢復(fù);恢復(fù)光照50 d后,葉綠素a/b、葉片可溶性糖含量、部分光合酶基因和SPS表達(dá)等基本與對(duì)照水平一致,僅Rubisco大亞基1(RbcL1)表達(dá)仍低于對(duì)照。說(shuō)明長(zhǎng)時(shí)間弱光處理對(duì)葡萄葉片光合中心的損傷短期內(nèi)不可逆,部分光合酶和蔗糖代謝關(guān)鍵酶基因表達(dá)受到抑制,葉片光合能力下降,光合產(chǎn)物合成與積累減少,進(jìn)而導(dǎo)致了葡萄果實(shí)變小,品質(zhì)降低。
[Abstract]:In order to investigate the effect of low light stress on photosynthetic physiology of grape leaves, 4-year-old 'Jufeng' grape was treated with 70% shading for 20 days after flowering, and then returned to the level of unshaded plant (control). Fruit quality and photosynthetic parameters, chlorophyll fluorescence, chlorophyll content, leaf sugar content and components, photosynthetic enzyme gene and sucrose phosphate synthase gene expression were measured. The results showed that the content of soluble solids decreased, the titratable acid content increased, the anthocyanin content of pericarp decreased and the vertical diameter of fruit decreased after low light treatment. With the extension of low light time, chlorophyll b content increased, chlorophyll a / b value decreased, photosynthetic rate and photochemical efficiency decreased, soluble sugar content in leaf decreased, Rubisco, small subunit, PRKase,SPS1-2, decreased. The expression of SPS3 and other genes was inhibited. The photosynthetic rate, photochemical efficiency and soluble sugar content increased slightly, and the expression of photosynthetic enzyme gene was partially recovered. After 50 days of light restoration, chlorophyll a / b, soluble sugar content, partial photosynthetic enzyme gene and SPS expression were basically the same as those of the control, but the expression of Rubisco large subunit 1 (RbcL1) was still lower than that of the control. The results showed that the damage to the photosynthetic center of grape leaves was irreversible for a long time, the expression of some key enzymes of photosynthesis and sucrose metabolism was inhibited, the photosynthetic ability of leaves was decreased, and the synthesis and accumulation of photosynthetic products were decreased. In turn, the grape fruit became smaller and the quality decreased.
【作者單位】： 上海交通大學(xué)農(nóng)業(yè)與生物學(xué)院;
【基金】：國(guó)家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項(xiàng)資金項(xiàng)目(CARS-30-zp-07)
【分類號(hào)】：S663.1

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