【摘要】：黑素皮質(zhì)素受體-4(Melanocortin-4 Receptor,MC4R)在參與調(diào)節(jié)攝食行為和機(jī)體能量平衡中發(fā)揮重要作用,但其在生殖活動中的作用尚不明確。為此本實(shí)驗(yàn)以不同發(fā)情周期中的C57小鼠為對象,利用免疫熒光對MC4R神經(jīng)元在下丘腦的表達(dá)進(jìn)行定位。通過Western Blot確定MC4R在小鼠發(fā)情前期、發(fā)情期、發(fā)情后期和間情期的下丘腦和卵巢中蛋白表達(dá)變化。結(jié)果表明:MC4R神經(jīng)元廣泛分布于下丘腦第三腦室,發(fā)情期下丘腦中MC4R蛋白表達(dá)量顯著低于其他3個時期(P0.05);同時期卵巢中MC4R蛋白表達(dá)量顯著低于發(fā)情前期和發(fā)情后期(P0.05),發(fā)情后期MC4R蛋白表達(dá)量極顯著高于其他3個時期(P0.01)。這一結(jié)果闡明了小鼠在發(fā)情周期不同階段下丘腦和卵巢組織中MC4R的表達(dá)變化規(guī)律,為進(jìn)一步研究MC4R在小鼠生殖活動中的作用提供理論基礎(chǔ)。
[Abstract]:Melanin receptor 4 (Melanocortin-4 Receptor,MC4R) plays an important role in regulating feeding behavior and energy balance, but its role in reproductive activities is unclear. The expression of MC4R neurons in hypothalamus was detected by immunofluorescence in C57 mice in different estrous cycles. Western Blot was used to determine the expression of MC4R in hypothalamus and ovary of mice in preestrus, late estrus and interoestrus. The results showed that MC4R neurons were widely distributed in the third ventricle of the hypothalamus, and the expression of MC4R protein in the hypothalamus during estrus was significantly lower than that in the other three stages (P0.05). The expression of MC4R protein in ovary at the same period was significantly lower than that in early estrus and late estrus (P0.05), and the expression of MC4R protein in late estrus was significantly higher than that in other three stages (P0.01). These results illustrate the changes of MC4R expression in hypothalamus and ovary of mice at different stages of estrous cycle and provide a theoretical basis for further study of the role of MC4R in mouse reproductive activities.
【作者單位】： 內(nèi)蒙古農(nóng)業(yè)大學(xué)獸醫(yī)學(xué)院;內(nèi)蒙古農(nóng)業(yè)大學(xué)職業(yè)技術(shù)學(xué)院;
【基金】：國家自然科學(xué)基金(31160455) 內(nèi)蒙古自然科學(xué)基金(2014MS0323) 中國博士后科學(xué)基金(2013M541212)
【分類號】：S865.13

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