【摘要】：為了建立一種分子標記輔助育種技術體系以增加蛹蟲草雜交親和性配對的比例,以4個蛹蟲草菌株(GD1、JS2、SD2、中13)為供試親本,通過無性孢子分離方法獲得了8株單交配型的雜交親本,根據(jù)蛹蟲草交配型位點MAT-alpha和MAT-HMG中基因序列設計了分子標記并指導配制親和性組合,最終成功獲得了12個雜交菌株。在栽培試驗的基礎上,分析了12個雜交菌株的栽培周期、產(chǎn)量、活性成分含量(蟲草素和腺苷)等相關性狀,結果表明,蛹蟲草雜交菌株存在超親現(xiàn)象。本研究建立的這套以交配型基因作為分子標記輔助選育蛹蟲草新品種的高效雜交育種技術體系,將有助于促進蛹蟲草栽培產(chǎn)業(yè)的發(fā)展。
[Abstract]:In order to establish a molecular marker-assisted breeding system to increase the proportion of cross compatibility pairing of Cordyceps militaris, four strains of Cordyceps militaris (13 in GD1,JS2,SD2,) were used as test parents. Eight hybrids of single mating type were obtained by asexual spores isolation method. Molecular markers were designed according to the gene sequences of MAT-alpha and MAT-HMG in the mating type sites of Cordyceps militaris and the affinity combinations were prepared. Finally, 12 hybrid strains were successfully obtained. On the basis of cultivation experiment, the cultivation cycle, yield and active component content (Cordycepin and adenosine) of 12 hybrid strains were analyzed. The results showed that the cross strains of Cordyceps militaris had superparent phenomenon. In this study, a set of highly efficient hybrid breeding techniques using mating genes as molecular markers to assist selection of new Cordyceps militaris varieties will be helpful to promote the development of cultivation industry of Cordyceps militaris (Cordyceps militaris).
【作者單位】： 國家食用菌工程技術研究中心/農(nóng)業(yè)部南方食用菌資源利用重點實驗室/上海市農(nóng)業(yè)遺傳育種重點開放實驗室/上海市農(nóng)業(yè)科學院食用菌研究所;安徽科技學院生命科學學院;
【基金】：上海市科委平臺建設項目(16DZ2281300)
【分類號】：S567.35

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