【摘要】：如果抗除草劑轉(zhuǎn)基因油菜的抗性基因滲入到野芥菜中,會給野芥菜的防除帶來很大困難。因此在抗除草劑轉(zhuǎn)基因油菜環(huán)境釋放前對抗性基因向野芥菜的滲入開展深入的研究非常必要。以抗草丁膦轉(zhuǎn)基因油菜與野芥菜的攜帶抗性基因回交3代子1代和子2代(BC3m F2和BC3p F2及BC3m F3和BC3p F3,m表示以野芥菜為母本的回交后代,p表示以野芥菜為父本的回交后代)為材料,在田間條件下研究了它們在不同密度(低密度為15株/區(qū),高密度為30株/區(qū))及不同種植比例(單種,野芥菜與回交后代以4∶1、3∶2、1∶1混種)時的適合度成分和總適合度。結(jié)果表明,無論是低密度還是高密度條件下,單種時BC3F2和BC3F3的總適合度均與野芥菜無顯著差異。低密度混種時,在4∶1和3∶2下,只有BC3m F3的總適合度與野芥菜無顯著差異,其余各后代的總適合度均顯著小于野芥菜;以1∶1混種時,只有BC3m F2和BC3m F3的總適合度與野芥菜無顯著差異。高密度混種時,3個比例混種下4種供試回交后代的總適合度均顯著小于野芥菜。相關性分析結(jié)果表明,BC3m F3的各適合度成分都與混種比例不相關。表明攜帶抗性基因的BC3F2和BC3F3在野外都具有生存定植的可能性,且BC3m F3定植的可能性較其他供試回交后代更大。因此在防范轉(zhuǎn)基因油菜基因逃逸的策略上,在防范初始雜交發(fā)生的同時,也應該防范回交后代的產(chǎn)生。
[Abstract]:If herbicide resistant genes of transgenic rapeseed infiltrate into wild mustard, it will bring great difficulty to the control of wild mustard. Therefore, it is necessary to study the penetration of resistance genes into wild mustard before herbicide resistant transgenic rapeseed is released. Transgenic rapeseed resistant to butylphosphine was used to backcross the first and second generations (BC3m F2 and BC3p F2 and BC3m F 3 and BC3p F 3) of the third generation (BC3m F2 and BC3p F2 and BC3m F3 and BC3p F3) to indicate the backcross progenies with wild mustard as the female parent. P the backcross progenies with wild mustard as male parent were used to study their different densities (low density 15 plants / region, high density 30 plants / region) and different planting ratios (single species) under field conditions. The suitable composition and total fitness of wild mustard and backcross progenies were 4: 1: 3: 2: 1: 1). The results showed that there was no significant difference in the total fitness of BC3F2 and BC3F3 between wild mustard and BC3F2 under low or high density. At 4:1 and 3:2, there was no significant difference between the total fitness of BC3m F3 and that of wild mustard, and the total fitness of other progenies was significantly lower than that of wild mustard. When mixed at 1:1, only BC3m F2 and BC3m F 3 had no significant difference between the total fitness of wild mustard and that of BC3m F2 and BC3m F 3. When mixed with high density, the total fitness of four backcross progenies under three proportion hybrids was significantly lower than that of wild mustard. The results of correlation analysis showed that all suitable components of BC3m F _ 3 were not related to the proportion of hybrid species. The results showed that both BC3F2 and BC3F3 carrying resistance genes had the possibility of survival and colonization in the field, and the probability of BC3m F3 colonization was higher than that of other backcross progenies. Therefore, in order to prevent the escape of transgenic rapeseed gene, we should prevent the generation of backcross progeny as well as the initial hybridization.
【作者單位】： 南京農(nóng)業(yè)大學雜草研究室;
【基金】：轉(zhuǎn)基因生物新品種培育科技重大專項子課題(2016ZX08012005-006) 國家自然科學基金項目(31270579)資助
【分類號】：S451;S565.4

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