水稻G蛋白γ亞基基因RGG2調控株型和抗旱性的研究
發(fā)布時間：2018-11-23 19:18
【摘要】：異三聚體G蛋白(Heterotrimeric G protein),是由a,p和γ三個亞單位構成的蛋白質復合物,它是生物體內一類重要的信號蛋白,參與多個信號響應過程。水稻中共有4個G蛋白γ亞基,分別為RGG1、RGG2、GS3和qPE9-1/DEP1,其中GS3是一個控制籽粒大小的主效QTL,它在調節(jié)籽粒和器官大小中發(fā)揮負調節(jié)子的功能；而qPE9-1是水稻直立穗形成的關鍵基因,同時參與水稻氮素和ABA的響應。而另外兩個G蛋白γ亞基RGGI和RGG2尚未進行詳細的功能分析。本研究以RGG2為研究對象,對該基因進行了表達分析、亞細胞定位和組織形態(tài)學等分析,初步揭示了RGG2的生物學功能。主要結果如下：1、RGG2屬于B型G蛋白γ亞基,與RGB1之間存在明顯互作；2、RGG2基因為組成型表達,且其表達水平比水稻其它3個G蛋白γ亞基基因RGG1、GS3和qPE9-1高出10倍以上。亞細胞定位結果顯示,RGG2蛋白位于細胞核、細胞質和細胞膜上；3、過量表達RGG2基因導致植株矮化,穗和葉片變短、直立,籽粒短圓,粒重減少,單株產量下降。說明RGG2是這些性狀的負調控因子;4、掃描電鏡和組織解剖分析表明,RGG2過量表達通過抑制細胞縱向伸長,而使得籽粒變小、節(jié)間變短,但不影響細胞數目。細胞分裂相關基因的表達水平在野生型和轉基因株系中差異很小,也從側面驗證了這一結論；5、觀察了日本晴和RGG2過量表達株系在ABA脅迫下的種子萌發(fā)與幼苗生長情況,發(fā)現(xiàn)RGG2過量表達系對ABA的響應顯著增強：6.干旱處理表明,RGG2過量表達株系葉片離體失水率和干旱脅迫下的植株死亡率均顯著低于對照。以上結果說明,RGG2作為G蛋白γ亞基,不僅調控水稻株型、粒型,還參與水稻對ABA及抗旱脅迫響應。
[Abstract]:Heterotrimer G protein (Heterotrimeric G protein),) is a protein complex composed of three subunits of ACP and 緯. It is a kind of important signal protein in organism and participates in many signal response processes. There are four G protein 緯 subunits in rice, RGG1,RGG2,GS3 and qPE9-1/DEP1, respectively. GS3 is a major QTL, that controls grain size. It plays a negative role in regulating grain and organ size. QPE9-1 is the key gene of rice panicle formation, and it is involved in the response of rice nitrogen and ABA. The other two G protein 緯 subunits, RGGI and RGG2, have not been analyzed in detail. In this study, the expression, subcellular localization and histomorphology of RGG2 gene were analyzed, and the biological function of RGG2 was preliminarily revealed. The main results are as follows: 1RGG2 belongs to type B G protein 緯 subunit, and there is obvious interaction between RGG2 and RGB1; (2) the expression level of RGG2 gene was 10 times higher than that of other three G protein 緯 subunit genes, RGG1,GS3 and qPE9-1. The results of subcellular localization showed that RGG2 protein was located in nucleus, cytoplasm and cell membrane, and 3, overexpression of RGG2 gene resulted in plant dwarfing, short panicle and leaf, erect, short round grain, reduced grain weight and decreased yield per plant. The results showed that RGG2 was a negative regulatory factor for these traits, and (4) scanning electron microscopy and histological analysis showed that RGG2 overexpression decreased the grain and shortened the internodes, but did not affect the number of cells by inhibiting the lengthwise elongation of the cells. The expression level of cell division related genes in wild type and transgenic lines was very small, and this conclusion was verified from the side. 5. Seed germination and seedling growth of Nippon and RGG2 overexpression lines under ABA stress were observed. It was found that the response of RGG2 overexpression lines to ABA was significantly enhanced: 6. Drought treatment showed that the rate of water loss in leaves of RGG2 overexpression lines and the plant mortality under drought stress were significantly lower than those of the control. These results suggest that RGG2, as a G protein 緯 subunit, not only regulates the plant type and grain type of rice, but also participates in the response of rice to ABA and drought stress.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S511
本文編號：2352465
[Abstract]:Heterotrimer G protein (Heterotrimeric G protein),) is a protein complex composed of three subunits of ACP and 緯. It is a kind of important signal protein in organism and participates in many signal response processes. There are four G protein 緯 subunits in rice, RGG1,RGG2,GS3 and qPE9-1/DEP1, respectively. GS3 is a major QTL, that controls grain size. It plays a negative role in regulating grain and organ size. QPE9-1 is the key gene of rice panicle formation, and it is involved in the response of rice nitrogen and ABA. The other two G protein 緯 subunits, RGGI and RGG2, have not been analyzed in detail. In this study, the expression, subcellular localization and histomorphology of RGG2 gene were analyzed, and the biological function of RGG2 was preliminarily revealed. The main results are as follows: 1RGG2 belongs to type B G protein 緯 subunit, and there is obvious interaction between RGG2 and RGB1; (2) the expression level of RGG2 gene was 10 times higher than that of other three G protein 緯 subunit genes, RGG1,GS3 and qPE9-1. The results of subcellular localization showed that RGG2 protein was located in nucleus, cytoplasm and cell membrane, and 3, overexpression of RGG2 gene resulted in plant dwarfing, short panicle and leaf, erect, short round grain, reduced grain weight and decreased yield per plant. The results showed that RGG2 was a negative regulatory factor for these traits, and (4) scanning electron microscopy and histological analysis showed that RGG2 overexpression decreased the grain and shortened the internodes, but did not affect the number of cells by inhibiting the lengthwise elongation of the cells. The expression level of cell division related genes in wild type and transgenic lines was very small, and this conclusion was verified from the side. 5. Seed germination and seedling growth of Nippon and RGG2 overexpression lines under ABA stress were observed. It was found that the response of RGG2 overexpression lines to ABA was significantly enhanced: 6. Drought treatment showed that the rate of water loss in leaves of RGG2 overexpression lines and the plant mortality under drought stress were significantly lower than those of the control. These results suggest that RGG2, as a G protein 緯 subunit, not only regulates the plant type and grain type of rice, but also participates in the response of rice to ABA and drought stress.
【學位授予單位】：揚州大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：S511
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