【摘要】：為了探討藏山羊成纖維細(xì)胞生長因子10(fibroblast growth factor 10,FGF10)基因分子結(jié)構(gòu)特征,并闡明其在藏山羊不同器官組織中的表達(dá)情況,試驗采用逆轉(zhuǎn)錄PCR(reverse transcription-PCR,RTPCR)克隆該基因,并采用實(shí)時熒光定量PCR(qPCR)技術(shù)檢測FGF10基因在藏山羊各器官組織中的表達(dá)情況。結(jié)果表明:克隆獲得藏山羊FGF10基因序列長度為1 252 bp,包括完整CDS區(qū)642 bp(Gen Bank登錄號為KX530811),編碼213個氨基酸,蛋白質(zhì)分子質(zhì)量為23.78 ku,屬于疏水性不穩(wěn)定蛋白,且主要分布于細(xì)胞外。與山羊FGF10基因CDS區(qū)比對結(jié)果顯示,藏山羊FGF10基因序列存在一個核苷酸突變位點(diǎn),并導(dǎo)致該位點(diǎn)纈氨酸(Val)突變?yōu)楫惲涟彼?Ile)。組織表達(dá)結(jié)果顯示,FGF10 mRNA主要在藏山羊肺臟、脾臟和背最長肌中高表達(dá),而在心臟、肝臟和腎臟器官組織中表達(dá)水平較低。
[Abstract]:In order to investigate the molecular structure of the fibroblast growth factor 10 (fibroblast growth factor 10 FGF10 gene in Tibetan goats and to elucidate its expression in different organs of Tibetan goats, reverse transcription PCR (reverse transcription-PCR,RTPCR (RT PCR (reverse transcription-PCR,RTPCR) was used to clone the gene. The expression of FGF10 gene in organs and tissues of Tibetan goats was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the length of FGF10 gene of Tibetan goat was 1 252 bp, including the complete CDS region 642 bp (Gen Bank accession number was KX530811), encoding 213 amino acids, the molecular weight of protein was 23.78 ku, belongs to hydrophobic unstable protein. And mainly distributed outside cells. The comparison with the CDS region of goat FGF10 gene showed that there was a nucleotide mutation site in Tibetan goat FGF10 gene sequence, which led to the mutation of valine (Val) to isoleucine (Ile). The results of tissue expression showed that FGF10 mRNA was mainly expressed in lung, spleen and longissimus dorsi muscle of Tibetan goats, but low in heart, liver and kidney.
【作者單位】： 西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院;青藏高原動物遺傳資源保護(hù)與利用四川省重點(diǎn)實(shí)驗室;
【基金】：中央高�；究蒲袠I(yè)務(wù)費(fèi)專項資金項目(2016NY Z0045) 四川省“十三五”畜禽育種攻關(guān)項目(2016NZ0099-36) 四川省科技創(chuàng)新產(chǎn)業(yè)鏈?zhǔn)痉豆こ讨卮箜椖?2014NZ0003)
【分類號】：S827

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本文編號：2332369