【摘要】：目的通過觀察Ras基因表達(dá)蛋白和COX2—PGE2—P450arom正反饋環(huán)中三指標(biāo)在子宮腺肌病小鼠治療前后隨動情周期變化的表達(dá)差異,及其各組子宮腺肌病小鼠自身在位及異位內(nèi)膜上的表達(dá)差異,研究清濕化瘀法對子宮腺肌病小鼠可能的作用靶點,以探索清濕化瘀法治療子宮腺肌病的作用機(jī)制。方法選取8周齡ICR雌鼠,通過異體垂體移植術(shù)建立小鼠子宮腺肌病模型,采用高、中、低劑量的內(nèi)異康復(fù)片和孕三烯酮以及丹莪婦康煎膏分別給藥治療,灌胃治療3月后取樣,應(yīng)用組織病理學(xué)檢查、免疫組織化學(xué)染色法檢測不同周期在位和異位內(nèi)膜上的Ras和COX2、PGE2、P450arom的表達(dá)。結(jié)果 (1)手術(shù)操作過程對造模結(jié)果無影響(P0.01);(2)造模后與正常組相比,各組的在位和異位內(nèi)膜Ras和COX2—PGE2—P450arom的表達(dá)均升高(P0.01),且6月模型組顯著高于3月模型組(P0.01),說明Ras和COX2—PGE2—P450arom表達(dá)量在造模后隨病程進(jìn)展逐漸升高。(3)治療前、后各模型組同期異位內(nèi)膜上的Ras和COX2—PGE2—P450arom的表達(dá)量高于在位內(nèi)膜(P0.01)。(4)治療后,與6月模型組同期相比,內(nèi)異康復(fù)片高、中計量組,孕三烯酮組,丹莪婦康煎膏組的小鼠在位和異位內(nèi)膜的Ras和COX2—PGE2—P450arom的表達(dá)量均顯著降低(P0.01)。結(jié)論清濕化瘀法可能通過下調(diào)在位及異位內(nèi)膜Ras基因蛋白表達(dá)或抑制COX2—PGE2—P450arom正反饋作用而從某一條或多途徑達(dá)到治療子宮腺肌病的目的 。
[Abstract]:Objective to observe the difference of expression of Ras gene expression protein and COX2-PGE2-P450arom positive feedback loop with estrous cycle before and after treatment in mice with adenomyosis. In order to explore the mechanism of removing dampness and removing blood stasis in treating adenomyosis of uterus, the possible targets of removing dampness and removing stasis were studied in order to explore the mechanism of removing dampness and removing blood stasis in the treatment of adenomyosis. Methods eight week-old ICR female rats were selected to establish the model of adenomyosis by allografts of pituitary gland in mice. The mice were treated with high, middle and low doses of Neihekangfu tablets, pregnenotrienone and Dan'e Fukang decoction respectively, and the samples were taken after 3 months of oral administration. The expression of Ras and COX2,PGE2,P450arom in different cycles of eutopic and ectopic endometrium were detected by histopathology and immunohistochemical staining. Results (1) the procedure of operation had no effect on the results of modeling (P0.01). (2) the expression of Ras and COX2-PGE2-P450arom in eutopic and ectopic endometrium of each group was higher than that of normal group (P0.01), and the expression of Ras and COX2-PGE2-P450arom in 6-month model group was significantly higher than that in 3-month model group (P0.01). The results showed that the expression of Ras and COX2-PGE2-P450arom increased with the course of the model. (3) before treatment, the expression of Ras and COX2-PGE2-P450arom on ectopic endometrium in each model group was higher than that in eutopic endometrium (P0.01). (4). Compared with the model group in June, the expression of Ras and COX2-PGE2-P450arom in the eutopic and ectopic endometrium of the mice in the middle dose group, pregnancy trienone group and Dan'e Fukang decoction group were significantly decreased (P0.01). Conclusion the method of removing dampness and removing blood stasis may be used to treat adenomyosis by down-regulating the expression of Ras gene in eutopic and ectopic endometrium or inhibiting the positive feedback of COX2-PGE2-P450arom.
【作者單位】： 成都中醫(yī)藥大學(xué);成都中醫(yī)藥大學(xué)附屬醫(yī)院;
【基金】：國家自然科學(xué)基金(No.81273796)
【分類號】：R285.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 關(guān)永格;何昱雯;李坤寅;;芍藥甘草湯對子宮腺肌病細(xì)胞ERK1 mRNA/ERK2 mRNA的影響[J];四川中醫(yī);2014年07期

2 朱萱萱;時燕平;王瓊;符蕾;張迪;張學(xué)非;;辛桂止痛栓對子宮腺肌病痛經(jīng)的實驗研究[J];中華中醫(yī)藥學(xué)刊;2014年07期

3 凌靜;關(guān)永格;李坤寅;王帥;;加味芍藥甘草湯對人子宮腺肌病病灶細(xì)胞超微結(jié)構(gòu)及細(xì)胞凋亡的影響[J];廣州中醫(yī)藥大學(xué)學(xué)報;2014年04期

相關(guān)博士學(xué)位論文 前2條

1 范為之;加味芍藥甘草湯對子宮腺肌病裸鼠miR-27a調(diào)控的P53/EGFR/STAT3通路的干預(yù)機(jī)制[D];廣州中醫(yī)藥大學(xué);2017年

2 侯曉;活血消ve方對子宮腺肌病模型大鼠Th1/Th2漂移及細(xì)胞凋亡機(jī)制的影響研究[D];山東中醫(yī)藥大學(xué);2014年

，

本文編號：2329619