產(chǎn)吲哚金黃桿菌YM5毒性相關(guān)基因及其急性經(jīng)口毒性
發(fā)布時(shí)間：2018-11-12 08:52
【摘要】：產(chǎn)吲哚金黃桿菌(Chryseobacterium indologenes)YM5是本實(shí)驗(yàn)室分離所得的一株具有高效羽毛角蛋白降解能力的革蘭氏陰性菌,具有潛在的工業(yè)應(yīng)用價(jià)值,但部分產(chǎn)吲哚金黃桿菌的相關(guān)株系被報(bào)道可能為醫(yī)院條件致病菌.本研究通過測(cè)定YM5全基因組序列,分析其基因組中毒性相關(guān)功能基因,結(jié)合小鼠急性經(jīng)口毒性試驗(yàn),來評(píng)價(jià)YM5的生物安全性.結(jié)果顯示,YM5基因組內(nèi)不含毒性相關(guān)基因.YM5菌液及其羽毛發(fā)酵液對(duì)小鼠半數(shù)致死量(LD50)大于20 g/kg BW,屬實(shí)際無毒級(jí);處理14 d后,小鼠血清中谷丙轉(zhuǎn)氨酶(AST)、谷草轉(zhuǎn)氨酶(ALT)、尿素(BUN)、肌酐(Cre)、血糖(GLU)、總膽固醇(TC)和甘油三酯(TG)與對(duì)照組均無顯著性差異(P0.05).本研究表明YM5菌液及其羽毛發(fā)酵液對(duì)哺乳動(dòng)物安全無毒副作用,可應(yīng)用于工業(yè)生產(chǎn).
[Abstract]:Clostridium indole producing (Chryseobacterium indologenes) YM5 is a Gram-negative strain with high degradation ability of feather keratin, which has potential industrial application value. However, some strains of Clostridium indole-producing bacteria were reported to be pathogenic bacteria in hospital. The purpose of this study was to evaluate the biological safety of YM5 by sequencing its entire genome, analyzing its genome-wide toxic related functional genes, and combining with acute oral toxicity test in mice. The results showed that there were no toxic genes in the YM5 genome. The half lethal dose (LD50) of YM5 and its feather fermentation broth was more than 20 g/kg BW,. After treatment for 14 days, serum alanine aminotransferase (AST), glutamic oxaloacetic transaminase (ALT), urea (BUN), creatinine (Cre), blood glucose (GLU),) were obtained in mice. There was no significant difference in total cholesterol (TC) and triglyceride (TG) between the control group and the control group (P0.05). This study showed that YM5 and its feather fermentation broth were safe and non-toxic to mammals, and could be used in industrial production.
【作者單位】： 中國(guó)科學(xué)院成都生物研究所;中國(guó)科學(xué)院大學(xué);中國(guó)科學(xué)院環(huán)境與應(yīng)用微生物重點(diǎn)實(shí)驗(yàn)室;
【基金】：中國(guó)科學(xué)院環(huán)境與應(yīng)用微生物重點(diǎn)實(shí)驗(yàn)室開放基金(KLCAS-2016-02,Y1C5101120)資助~~
【分類號(hào)】：Q933
本文編號(hào)：2326639
[Abstract]:Clostridium indole producing (Chryseobacterium indologenes) YM5 is a Gram-negative strain with high degradation ability of feather keratin, which has potential industrial application value. However, some strains of Clostridium indole-producing bacteria were reported to be pathogenic bacteria in hospital. The purpose of this study was to evaluate the biological safety of YM5 by sequencing its entire genome, analyzing its genome-wide toxic related functional genes, and combining with acute oral toxicity test in mice. The results showed that there were no toxic genes in the YM5 genome. The half lethal dose (LD50) of YM5 and its feather fermentation broth was more than 20 g/kg BW,. After treatment for 14 days, serum alanine aminotransferase (AST), glutamic oxaloacetic transaminase (ALT), urea (BUN), creatinine (Cre), blood glucose (GLU),) were obtained in mice. There was no significant difference in total cholesterol (TC) and triglyceride (TG) between the control group and the control group (P0.05). This study showed that YM5 and its feather fermentation broth were safe and non-toxic to mammals, and could be used in industrial production.
【作者單位】： 中國(guó)科學(xué)院成都生物研究所;中國(guó)科學(xué)院大學(xué);中國(guó)科學(xué)院環(huán)境與應(yīng)用微生物重點(diǎn)實(shí)驗(yàn)室;
【基金】：中國(guó)科學(xué)院環(huán)境與應(yīng)用微生物重點(diǎn)實(shí)驗(yàn)室開放基金(KLCAS-2016-02,Y1C5101120)資助~~
【分類號(hào)】：Q933
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