【摘要】：本試驗旨在研究夏季高溫條件下飼糧中添加刺梨提取物對皖西白鵝生長性能、脂質代謝及肝臟中熱應激蛋白70(HSP70)基因表達的影響。挑選28日齡、體重相近的健康皖西白鵝150只,隨機分成3組,即對照組、試驗1組和試驗2組,每組5個重復,每個重復10只鵝。對照組試驗鵝飼喂基礎飼糧,試驗1組和試驗2組試驗鵝分別飼喂在基礎飼糧中添加100和200 mL/kg刺梨提取物的試驗飼糧。預試期7 d,正試期14 d,試驗期間最高氣溫37℃,最低氣溫24℃,日平均氣溫31.6℃。飼喂2周后,每個重復挑選1只鵝屠宰,采集血樣,并分離出肝臟,測定血清脂質代謝指標和肝臟中HSP70基因的相對表達量。結果表明:飼糧中添加刺梨提取物對皖西白鵝的平均日采食量和平均日增重均無顯著影響(P0.05),但添加200 mL/kg刺梨提取物可顯著降低料重比(P0.05);飼糧中添加100和200 mL/kg刺梨提取物均可顯著提高血清高密度脂蛋白含量(P0.05),但對血清總膽固醇和甘油三酯含量無顯著影響(P0.05);與對照組相比,試驗1組和試驗2組肝臟中HSP70基因的相對表達量均極顯著提高(P0.01),同時試驗1組肝臟中HSP70基因的相對表達量還極顯著高于試驗2組(P0.01)。由此得出,在夏季高溫條件下,在皖西白鵝飼糧中添加刺梨提取物可提高飼料轉化率、調節(jié)脂質代謝、上調肝臟中HSP70基因的表達,具有較明顯的抗應激效果,且添加量為100 mL/kg時的抗應激效果優(yōu)于添加量為200 mL/kg時。
[Abstract]:The purpose of this study was to study the effects of the extract of Rosa roxburghii on growth performance, lipid metabolism and heat stress protein 70 (HSP70) gene expression in liver of Wanxi White Goose under high temperature in summer. One hundred and fifty healthy Wanxi white geese of similar weight and 28 days of age were selected and randomly divided into three groups: control group, test group 1 and test group 2 with 5 replicates in each group and 10 geese in each group. The experimental geese in the control group were fed with basic diet, the experimental geese in group 1 and group 2 were fed with 100 and 200 mL/kg extract of Rosa roxburghii in the basal diet, respectively. The maximum temperature was 37 鈩，

本文編號：2326885