Advance in Application of Transgenic Breeding Technology in
本文關(guān)鍵詞:轉(zhuǎn)基因育種技術(shù)在菊花性狀改良中的應(yīng)用進(jìn)展,由筆耕文化傳播整理發(fā)布。
轉(zhuǎn)基因育種 菊花性狀改良
園 藝 學(xué) 報(bào) 2013,40(9):1813–1825 http: // ahs. ac. cn Acta Horticulturae Sinica E-mail: yuanyixuebao@ 轉(zhuǎn)基因育種技術(shù)在菊花性狀改良中的應(yīng)用進(jìn)展 成麗娜,魏 倩,Muhammad Imtiaz,高俊平,洪 波*
(中國農(nóng)業(yè)大學(xué)觀賞園藝與園林系,北京 100193)
摘 要:自1989年發(fā)現(xiàn)了菊花對(duì)農(nóng)桿菌浸染的敏感性,農(nóng)桿菌介導(dǎo)轉(zhuǎn)化法已成功應(yīng)用于菊花花色、
花期、株形、抗病蟲及耐非生物脅迫等性狀的改良中。從影響菊花遺傳轉(zhuǎn)化效率的農(nóng)桿菌菌株類型、外
植體選用、輔助添加劑的使用以及抗生素篩選等幾方面闡述了菊花遺傳轉(zhuǎn)化技術(shù)研究的進(jìn)展和應(yīng)用現(xiàn)狀,對(duì)菊花分子育種性狀改良和重要功能基因發(fā)掘等研究進(jìn)展進(jìn)行綜述,并對(duì)菊花轉(zhuǎn)化效率改善途徑的探索
和環(huán)境友好型轉(zhuǎn)基因新技術(shù)研發(fā)前景提出展望,以期為菊花分子育種提供參考。
關(guān)鍵詞:菊花;農(nóng)桿菌;轉(zhuǎn)基因;分子育種;性狀改良
中圖分類號(hào):S 682.1+1 文獻(xiàn)標(biāo)志碼:A 文章編號(hào):0513-353X(2013)09-1813-13
Advances in Application of Transgenic Breeding Technology in the Traits Improvement of Chrysanthemum
CHENG Li-na,WEI Qian,Muhammad Imtiaz,GAO Jun-ping,and HONG Bo*
(Department of Ornamental Horticulture,China Agricultural University,Beijing 100193,China)
Abstract:Following the discovery of chrysanthemum sensitivity to Agrobacterium in 1989,the technique of Agrobacterium-mediated genetic transformation has been successfully used in improvement of traits such as flower color,plant style,flowering time,,disease-insect resistance and abiotic stress tolerance. In this review,the advances of Agrobacterium-mediated genetic transformation in chrysanthemum were summarized,including Agrobacterium strains,explants for infection,the addition of chemical compounds and selection markers. Also,the improvement of traits,exploration of important genes was reviewed and the perspective of enhancing transformation efficiency and developing environment-friendly transgenic technology were discussed. The main goal is to provide reference for molecular breeding of chrysanthemum.
Key words:chrysanthemum;Agrobaterium;transgenic technology;molecular breeding;improvement of traits
通過常規(guī)的雜交育種手段,菊花[Chrysanthemum morifolium(Ramat.)Tzvel)]的品種改良已在株形、花色、花期、抵抗病蟲害和非生物脅迫等方面取得了顯著的成績。但菊花高度雜合的特點(diǎn)使其在雜交育種中不確定性很強(qiáng),生殖或地理隔離使其無法充分利用近緣種屬植物的優(yōu)良基因資源,常規(guī)方法育種存在較大的局限性。轉(zhuǎn)基因育種目前已成為菊花新品種培育的重要手段,外源基因的導(dǎo)入有針對(duì)性地改善了目標(biāo)性狀并保留原有優(yōu)良性狀,實(shí)現(xiàn)了利用新基因資源改良菊花品種的目標(biāo)。 收稿日期:2013–06–21;修回日期:2013–08–20
基金項(xiàng)目:北京市園林綠化局計(jì)劃項(xiàng)目(YLHH201300104)
*通信作者 Author for correspondence(E-mail:hongbo1203@)
本文關(guān)鍵詞:轉(zhuǎn)基因育種技術(shù)在菊花性狀改良中的應(yīng)用進(jìn)展,由筆耕文化傳播整理發(fā)布。
本文編號(hào):232441
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