本文關(guān)鍵詞：基因槍法獲得玉米轉(zhuǎn)基因植株的研究，由筆耕文化傳播整理發(fā)布。

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transgenic maize

    SCMV-resistant Transgenic Maize Obtained by Using Different Strategies

    利用不同策略獲得抗SCMV轉(zhuǎn)基因玉米的研究

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    EXPRESSION ACTIVITY OF MAIZE Ubi-1 PROMOTER IN FERTILE TRANSGENIC MAIZE PLANTS

    玉米Ubi-1啟動(dòng)子在可育轉(zhuǎn)基因玉米植株中的表達(dá)活性

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    The Research and Application Transgenic Maize

    轉(zhuǎn)基因玉米的研究與應(yīng)用

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    Effects of the Foreign Gene Encoding a Lysine Rich Protein on Protein Compositions in Transgenic Maize Grain

    外源高賴(lài)氨酸蛋白質(zhì)基因?qū)D(zhuǎn)基因玉米種子中蛋白質(zhì)組分的影響

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    Specific Detection of Alien DNA Sequences in Six Transgenic Maize by Using Multiplex PCR Method

    用復(fù)合PCR方法檢測(cè)6種轉(zhuǎn)基因玉米中外源DNA的特異性

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    Developments in Transgenic Maize

    玉米轉(zhuǎn)基因研究進(jìn)展

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    Research and Developments in Transgenic Maize

    玉米轉(zhuǎn)基因研究與進(jìn)展

短句來(lái)源

    Study on the Development of Transgenic Maize plants by Bombardment Transformation

    基因槍法獲得玉米轉(zhuǎn)基因植株的研究

    In this paper, the author reviewed the research advances of transgenic maize in transfergenic receptor system,target genes and transformation methods. Further, this paper briefly introduced the questions and ways of transgenic maize and the advances on other types maize in recent years

    在此,就玉米轉(zhuǎn)基因在受體系統(tǒng)、目的基因及轉(zhuǎn)化方法方面的研究成果,尤其是近年來(lái)玉米轉(zhuǎn)基因研究方面存在的問(wèn)題、解決途徑以及其他類(lèi)型玉米的轉(zhuǎn)基因研究進(jìn)展做了綜述。

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    In this paper,the new research and developments made in various aspects of transgenic maize was summarized,including selection of receptor,methods and theory of transformation,selection of marker genes and promoters,establishment of expression vector,aiming characters and the prospect of application.

    從玉米轉(zhuǎn)基因的受體選擇、遺傳轉(zhuǎn)化方法和原理、標(biāo)記基因的選擇、表達(dá)載體構(gòu)建和啟動(dòng)子的選擇及玉米轉(zhuǎn)基因研究、生產(chǎn)和應(yīng)用前景等方面,闡述了玉米轉(zhuǎn)基因研究的進(jìn)展。

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    The mortalities of the 1st to 3rd instar larvae feeding on Bt transgenic maize for 1～2 days were up to 90%,and no death occurred in the 4th instar larvae,but the growth decreased.

    1-3齡幼蟲(chóng)取食轉(zhuǎn)B t基因玉米后1-2 d死亡,死亡率達(dá)90%以上; 4齡以后死亡率為0,但其生長(zhǎng)發(fā)育均受到一定的抑制;

短句來(lái)源

    The result showed that integration of foreign gene Fe- SOD into the genome of the TO transgenic maize plants was confirmed by PCR、PCR-Southern blot and RT-PCR and the gene was expressed at the transcirption level.

    PCR、PCR-Southern、RT-PCR分子檢測(cè)結(jié)果表明:Fe-SOD基因被整合到玉米基因組中,并在轉(zhuǎn)錄水平上表達(dá)。

    Bio-assays and tissue section examinations were conducted for determining the resistance of Bt transgenic maize N3030Bt to Ostrinia fumacalis.

    以普通玉米雜交組合N3030為對(duì)照,通過(guò)田間調(diào)查、盆栽接蟲(chóng)試驗(yàn)、室內(nèi)飼養(yǎng)和組織切片,系統(tǒng)研究了轉(zhuǎn)Bt基因玉米雜交組合N3030Bt對(duì)亞洲玉米螟的抗性表現(xiàn)。

    Cloning of a Lysine-rich Gene SBgLR and the Effect of Improving the Protein and Lysine Content in Transgenic Maize Seed

    高賴(lài)氨酸蛋白基因SBgLR的克隆及其對(duì)提高玉米種子中蛋白質(zhì)和賴(lài)氨酸含量的作用

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    Production and Analysis of Transgenic Maize with Improved Salt Tolerance by the Introduction of AtNHX1 Gene

    轉(zhuǎn)AtNHX1基因玉米的產(chǎn)生及其耐鹽性分析(英文)

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  transgenic maize

Results of Southern and Western blotting analysis certified that this gene had integrated and been expressed in transgenic maize plants and their progeny.

Ten percent of the transgenic maize expressed single fusion proteins from the translational fusion gene cry1B-1Ab and showed resistance to these two pests as well as to the fall armyworm.

Transgenic maize plants that carried the cry1B gene in T1 to T3 progenies transmitted trangenes with expected Mendelian segregation and conferred resistance to the two target insects.

Expression and inheritance of the wheat Glu-1DX5 gene in transgenic maize

We have produced transgenic maize plants containing a wheat Glu-1Dx5 gene encoding the high-molecular-weight glutenin subunit 1Dx5.

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Several maize inbreds were transformed with Agrobacterium tumefaciens EHA101 (pGIH). Transgenic maize plants were obtained. Frequency of transformation of maize inbred Suyu No. 1 can reach 8. 1%. Results of PCR and Southern blot analysis proved that T-DNA was stably integrated into the genome of maize. Staining with X-gluc confirmed the expression of GUS gene in maize cells. The band amplified by inverse PCR

用帶有質(zhì)粒pGIH(35S-intron-GUS/ Hptr)的根癌農(nóng)桿菌EHA101轉(zhuǎn)化玉米愈傷組織,獲得潮霉素抗性植株,再生性好的蘇玉1 圖7 Southern blot分析HpaII消化的質(zhì)粒pGIH 和gusA基因沉默的愈傷組織的總DNA Fig.7 Southern blot analysis of plasmid pGIH(P) and plant genomic DNA of gusA gene si- lence callus(T)digested with HpaII號(hào)轉(zhuǎn)化率可以達(dá)到8.1%。對(duì)轉(zhuǎn)化植株進(jìn)行GUS染色分析及PCR和Southern雜交檢測(cè)證明外源基因已經(jīng)整合,并能夠穩(wěn)定表達(dá)。反向PCR分析的三個(gè)轉(zhuǎn)化植株,T-DNA插入片段均為單拷貝。部分失去分化能力的抗性愈傷組織,Southern blot分析發(fā)現(xiàn)其基因組中有g(shù)usA基因插入,但X-gluc染色呈陰性,經(jīng)HpaII酶切分析發(fā)現(xiàn)整合的gusA基因發(fā)生了高度甲基化。

Foreign insecticidal genes were transgerred into 2 elite inbred lines of maize (Zea mays L.) by microprojectile bombardment. It was found that a series of measures could effectively reduce clonal variation and elevate the rate of viability of transgenic maize during the processes of calli receptor preparation, selection, regeneration and planting.

通過(guò)基因槍轉(zhuǎn)化法將外源抗蟲(chóng)基因?qū)?1個(gè)玉米 (ZeamaysL .)優(yōu)良自交系。對(duì)受體愈傷的制備 ,抗性愈傷的篩選分化和轉(zhuǎn)基因植株的移栽等方面的研究 ,采用一系列的措施 ,有效地降低了轉(zhuǎn)化植株的畸形率 ,提高了轉(zhuǎn)化植株的成活率

s:Techniques such as Agrobacteria,particulate gun and PEG inducing techniques are often adopted in maize transgenic research,While vigorous part such as callus and yong embryo typically are used as acceptance.Transgenic Bt maize and transgenic herbicide-resistant maize are successfully developed.World wide conscious to environmental pollution protection and feed chains remains obstacle to Transgenic maize application.

玉米轉(zhuǎn)基因的方法主要有農(nóng)桿菌、基因槍、PEG介導(dǎo)等方法 ,受體主要是愈傷組織和幼胚等生活較旺盛的部位。目前在玉米上的成功應(yīng)用是抗玉米螟的Bt轉(zhuǎn)基因玉米、抗除草劑轉(zhuǎn)基因玉米等種類(lèi)。轉(zhuǎn)基因技術(shù)目前面臨的問(wèn)題是關(guān)于環(huán)境和食物鏈的問(wèn)題。也就未來(lái)玉米的轉(zhuǎn)基因發(fā)展進(jìn)行了討論。

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  本文關(guān)鍵詞：基因槍法獲得玉米轉(zhuǎn)基因植株的研究，由筆耕文化傳播整理發(fā)布。