【摘要】：目的了解鴨肉生產(chǎn)鏈中彎曲菌的污染情況及其耐藥性和毒力基因分布情況。方法根據(jù)GB 4789.9-2014,從肉鴨屠宰鏈中分離疑似彎曲菌,采用三重PCR方法,對(duì)疑似菌株進(jìn)行準(zhǔn)確鑒定;采用微量肉湯稀釋法,對(duì)彎曲菌分離菌株進(jìn)行8種抗菌藥物敏感性試驗(yàn),參考NARMS標(biāo)準(zhǔn)判定藥敏試驗(yàn)結(jié)果;利用PCR檢測與彎曲菌致病相關(guān)的4個(gè)毒力基因。結(jié)果根據(jù)形態(tài)特征、生化指標(biāo)及PCR鑒定結(jié)果,從489份樣品中,鑒定出空腸彎曲菌100株、結(jié)腸彎曲菌79株,其他彎曲菌8株,彎曲菌的總體分離率為38.24%,肉鴨屠宰前、脫毛環(huán)節(jié)、掏膛環(huán)節(jié)、屠宰后樣品彎曲菌分離率分別為76.33%、5.62%、24.00%、0%;藥敏試驗(yàn)結(jié)果表明彎曲菌對(duì)TET(95.72%)、CLI(90.91%)的耐藥率較高,對(duì)AZI(63.64%)耐藥率居中,對(duì)CIP(31.02%)、GEN(34.76%)、NAL(37.43%)、ERY(41.18%)、CHL(41.18%)的耐藥率相對(duì)較低,所分離菌株多重耐藥現(xiàn)象較為普遍,多重耐藥率達(dá)72.19%。彎曲菌分離株對(duì)黏附相關(guān)基因cadF、鞭毛基因flaA、侵襲蛋白基因iamA、毒素調(diào)節(jié)基因cdtB的攜帶率分別為100%、80.75%、71.12%、94.65%。結(jié)論肉鴨屠宰過程中存在不同程度的彎曲菌污染,且其耐藥情況較為嚴(yán)重,毒力相關(guān)基因廣泛存在于彎曲菌中,應(yīng)該加強(qiáng)衛(wèi)生管理和抗菌藥物使用監(jiān)督。
[Abstract]:Objective to investigate the contamination of Campylobacter in duck production chain and its resistance and virulence gene distribution. Methods according to GB 4789.9-2014, suspected campylobacter was isolated from meat duck slaughter chain, and the suspected strain was identified accurately by triple PCR method. Eight antimicrobial susceptibility tests were carried out on campylobacter isolates by broth dilution method, and four virulence genes related to campylobacter pathogenicity were detected by PCR with reference to NARMS criteria. Results according to the morphological characteristics, biochemical indexes and PCR results, 100 strains of Campylobacter jejuni, 79 strains of Campylobacter coli and 8 strains of other campylobacter were identified from 489 samples. The total isolation rate of Campylobacter was 38.24%. The isolation rate of Campylobacter after slaughter was 76.330.5.62% and 24.00%, respectively. The results of drug sensitivity test showed that Campylobacter campylobacter had higher resistance to TET (95.72%), CLI (90.91%), middle to AZI (63.64%) and CIP (31.02%), GEN (34.76%), NAL (37.43%). The drug resistance rate of ERY (41.18%), CHL (41.18%) was relatively low. The multidrug resistance of the isolated strains was relatively common, and the multidrug resistance rate reached 72.19%. The carrying rates of campylobacter isolates to adhesion gene cadF, flagellum gene flaA, invasion protein gene iamA, toxin regulatory gene cdtB were 100% 80.75% 71.1210% 94.65% respectively. Conclusion Campylobacter spp is contaminated in different degree during slaughtering, and its drug resistance is serious. Virulence related genes are widely found in campylobacter, so hygiene management and antimicrobial drug use supervision should be strengthened.
【作者單位】： 四川農(nóng)業(yè)大學(xué)食品學(xué)院;農(nóng)產(chǎn)品加工及貯藏工程四川省重點(diǎn)實(shí)驗(yàn)室;四川農(nóng)業(yè)大學(xué)都江堰校區(qū)微生物學(xué)實(shí)驗(yàn)室;四川農(nóng)業(yè)大學(xué)動(dòng)物醫(yī)學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(No.31400066) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)子課題(No.200903055)聯(lián)合資助~~
【分類號(hào)】：S852.61

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