【摘要】：目的了解和比較安徽農(nóng)村地區(qū)人類免疫缺陷病毒(human immunodeficiency virus,HIV)感染者和幼兒園兒童中藍(lán)氏賈第鞭毛蟲(Giardia lamblia)感染情況及其基因型。方法2015年4月24日至5月9日收集安徽農(nóng)村地區(qū)某艾滋病治療點(diǎn)登記在冊的HIV感染者及當(dāng)?shù)匾患矣變簣@所有兒童的相關(guān)信息,并采集糞樣進(jìn)行碘液染色及鏡檢。提取鏡檢陽性者的糞樣DNA,采用巢式PCR擴(kuò)增藍(lán)氏賈第鞭毛蟲磷酸丙糖異構(gòu)酶(triosephosphate isomerase,tpi)基因并測序,通過BLAST、Clustal X 1.83和MEGA 6.0軟件對基因序列進(jìn)行同源性比較和系統(tǒng)發(fā)育分析。結(jié)果共調(diào)查HIV感染者127人和兒童125人,發(fā)現(xiàn)賈第鞭毛蟲感染者4例,其中兒童3例,HIV感染者1例,感染率分別為2.40%(3/125)和0.79%(1/127),兩者差異無統(tǒng)計(jì)學(xué)意義(P0.05)。3例兒童感染者糞便為成形軟便,無腹瀉、腹痛等臨床癥狀;1例HIV感染者糞便為稀便,且有明顯腹痛、腹瀉、乏力癥狀,體重下降明顯。PCR檢測結(jié)果顯示,4例感染者糞樣DNA擴(kuò)增產(chǎn)物均在約500 bp處出現(xiàn)特異條帶。測序結(jié)果顯示,4例均感染藍(lán)氏賈第鞭毛蟲,其中HIV感染者2個(gè)重復(fù)樣品的序列相似性為79%,與已報(bào)道的上海人源賈第鞭毛蟲分離株(Gen Bank登錄號:KF271445)相似性分別為79%、98%;3名兒童感染者的樣本序列相似性達(dá)99%,與上海人源賈第鞭毛蟲分離株相似性均為79%。以tpi基因構(gòu)建的系統(tǒng)進(jìn)化樹結(jié)果顯示,HIV感染者的藍(lán)氏賈第鞭毛蟲分離株為復(fù)合型集聚體A+B,3名兒童感染者分離株均為集聚體A;HIV感染者的集聚體A與3名兒童感染者分離株的同源性較高。結(jié)論安徽農(nóng)村地區(qū)HIV感染者和兒童人群中存在藍(lán)氏賈第鞭毛蟲感染,HIV感染者的蟲株基因型為復(fù)合型集聚體A+B,兒童的蟲株基因型皆為集聚體A。
[Abstract]:Objective to investigate and compare the (Giardia lamblia) infection and genotype of Giardia lamblia in rural areas of Anhui province. Methods from April 24 to May 9, 2015, we collected the relevant information of HIV infected persons registered at an AIDS treatment site in rural Anhui and all children in a local kindergarten, and collected feces for iodine staining and microscopic examination. Fecal DNA, was extracted from positive individuals. Nested PCR was used to amplify and sequence the triosephosphate isomerase,tpi gene of Giardia lamblia. Clustal X 1.83 and MEGA 6.0 were used to compare the homology and phylogenetic analysis. Results A total of 127 cases of HIV infection and 125 cases of children were investigated. Four cases of Giardia lamblia infection were found, including 3 cases of children and 1 case of HIV infection. The infection rate was 2.40% (3 / 125) and 0.79% (1 / 127), respectively. There was no significant difference between the two groups (P0.05). The feces of 3 children infected with infection were soft stool, no diarrhea, abdominal pain and other clinical symptoms. The feces of one case of HIV infection were dilute stool with obvious abdominal pain, diarrhea, fatigue and weight loss. The results of PCR detection showed that the fecal DNA amplification products of 4 cases of HIV infected patients had specific bands at about 500 bp. The sequencing results showed that all the 4 cases were infected with Giardia lamblia. The sequence similarity of two repeat samples of HIV infection was 79, and the similarity was 79 with the reported (Gen Bank accession number: KF271445 of Shanghai Giardia lamblia isolates. 98. The similarity of the three infected children was 99g, and the similarity with Shanghai Giardia flagellate isolate was 79g. The results of phylogenetic tree constructed by tpi gene showed that the isolates of Giardia lambtii infected with HIV were compound agglomeration A BU. All the isolates of 3 children infected with HIV were agglomerates. There was a high homology between the agglomeration A of HIV infection and the isolated strains of 3 children infected with HIV. Conclusion there is Giardia lamblia infection in HIV infected persons and children in rural areas of Anhui province. The genotype of HIV infected individuals is compound agglomeration A B, and that of children is agglomeration A.
【作者單位】： 中國疾病預(yù)防控制中心寄生蟲病預(yù)防控制所 世界衛(wèi)生組織熱帶病合作中心 科技部國家級熱帶病國際聯(lián)合中心 衛(wèi)生部寄生蟲病原與媒介生物學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家自然科學(xué)基金(No.81473022) 上海市衛(wèi)計(jì)委科研課題(No.140826120622123)~~
【分類號】：R725

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