【摘要】：近年來人們在十字花科物種系統(tǒng)發(fā)生關(guān)系方面開展了大量工作,研究發(fā)現(xiàn)十字花科可分為3個主要類群,但是這些類群內(nèi)部以及類群間的進(jìn)化關(guān)系還不明確。旨在快速準(zhǔn)確地解決十字花科物種系統(tǒng)發(fā)生關(guān)系,通過選取39個十字花科物種及兩個外類群物種作為研究材料,使用系統(tǒng)發(fā)生基因組學(xué)方法獲得了覆蓋所選物種的低拷貝同源基因集合。進(jìn)一步通過CVTree方法分析低拷貝核基因的組分特征,得到了高度支持與穩(wěn)定的十字花科系統(tǒng)發(fā)育關(guān)系。結(jié)果顯示,十字花科被分為6個主要的類群,其中3個主要類群的劃分與前人的分類結(jié)果高度一致,并且增加了兩個新類群,此外,前人研究中存在爭議的第二類群在本研究結(jié)果中成為有穩(wěn)定支持的單系群。表明基于大量低拷貝同源基因集合并結(jié)合組分矢量分析,可以較為準(zhǔn)確地反映十字花科物種的系統(tǒng)發(fā)生關(guān)系。因此,CVTree方法不僅適用于研究原核生物、真菌等微生物的系統(tǒng)發(fā)生關(guān)系,也可以用來探究十字花科植物等高等生物的親緣關(guān)系。
[Abstract]:In recent years, a great deal of work has been done on the phylogenetic relationship of Cruciferae species. It is found that the Cruciferae can be divided into three main groups, but the evolutionary relationship within these groups and among them is not clear. In order to solve the phylogenetic relationship of Cruciferae species quickly and accurately, 39 species of Cruciferae and two species of exospecies were selected as the research materials. A set of low copy homologous genes covering selected species was obtained using phylogenetic genomics. Furthermore, the component characteristics of low copy nuclear genes were analyzed by CVTree method, and a highly supportive and stable phylogenetic relationship of cruciferous families was obtained. The results show that the Cruciferae is divided into six major taxa, and the classification of three major groups is highly consistent with the results of previous classification, and two new taxa are added. The second group, which has been controversial in previous studies, has become a stable monolithic group in the present study. It is shown that the phylogenetic relationship of cruciferous species can be accurately reflected based on a large number of low copy homologous gene sets and component vector analysis. Therefore, CVTree method can not only be used to study the phylogenetic relationship of prokaryotes, fungi and other microorganisms, but also to explore the genetic relationship of higher organisms such as cruciferous plants.
【作者單位】： 復(fù)旦大學(xué)生命科學(xué)學(xué)院植物科學(xué)研究所遺傳與發(fā)育協(xié)同創(chuàng)新中心;
【基金】：國家自然科學(xué)基金項目(91131007)
【分類號】：Q943.2
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本文編號：2312773