【摘要】：為了明確陸地棉開花促進(jìn)因子基因的作用,以中棉所36葉片基因組DNA和cDNA為材料克隆得到了開花相關(guān)基因Gh FLP1,分析了其特征及功能。該基因全長(zhǎng)為537 bp,包含339 bp開放閱讀框,編碼112個(gè)氨基酸。預(yù)測(cè)分子量約為12.176 kDa,理論等電點(diǎn)為9.13。組織特異性表達(dá)分析表明,該基因在花蕾中優(yōu)勢(shì)表達(dá),且在早熟品種(中棉所36、中棉所74)中表達(dá)量高于中熟品種(中棉所60、魯棉研28)。啟動(dòng)子序列分析和外源激素處理實(shí)驗(yàn)表明該基因受水楊酸和赤霉素調(diào)控。農(nóng)桿菌介導(dǎo)轉(zhuǎn)化擬南芥發(fā)現(xiàn),該基因能促使擬南芥蓮座葉減少,開花期提前。熒光定量結(jié)果表明,在轉(zhuǎn)基因擬南芥中,內(nèi)源開花相關(guān)基因AtFT、AtLFY、AtAP1和AtSOC1表達(dá)量不同程度上調(diào),AtFUL表達(dá)量基本不變,AtFLC表達(dá)量下調(diào)。研究結(jié)果顯示該基因可能參與陸地棉開花時(shí)間的調(diào)控,為創(chuàng)制轉(zhuǎn)基因早熟棉花新材料打下基礎(chǔ)。
[Abstract]:In order to clarify the role of flowering promoting factor gene in Upland cotton, the flowering related gene Gh FLP1, was cloned from the genomic DNA and cDNA of Zhongmianyi 36 leaves to analyze its characteristics and functions. The full length of the gene is 537 bp, which contains 339 bp open reading frame and encodes 112 amino acids. The predicted molecular weight is about 12.176 kDa, and the theoretical isoelectric point is 9.13. Tissue specific expression analysis showed that the expression of the gene was higher in early maturing varieties (Zhongmiansuo 36, Zhongmianyan 74) than in mid-maturing varieties (Zhongmiansuo 60, Lumianyan 28). Promoter sequence analysis and exogenous hormone treatment showed that the gene was regulated by salicylic acid and gibberellin. Agrobacterium tumefaciens mediated transformation of Arabidopsis thaliana showed that the gene could promote the decrease of rosette leaves and advance the flowering period. The results of fluorescence quantitative analysis showed that in transgenic Arabidopsis thaliana, the expression of endogenous flowering related gene AtFT,AtLFY,AtAP1 and AtSOC1 was up-regulated, the expression of AtFUL remained unchanged, and the expression of AtFLC was down-regulated. The results showed that the gene might be involved in the regulation of flowering time of upland cotton and lay the foundation for the creation of new transgenic early maturing cotton materials.
【作者單位】： 西北農(nóng)林科技大學(xué)農(nóng)學(xué)院;中國(guó)農(nóng)業(yè)科學(xué)院棉花研究所/棉花生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家科技支撐計(jì)劃(2014BAD03B01)
【分類號(hào)】：S562

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡銀崗;林凡云;王士強(qiáng);何蓓如;;糜子抗旱節(jié)水相關(guān)基因PmMYB的克隆及表達(dá)分析[J];遺傳;2008年03期

2 李順成,龐有志;天然基因克隆的制備[J];洛陽農(nóng)專學(xué)報(bào);1994年02期

3 劉羽;趙傳志;李長(zhǎng)生;李翠;王興軍;;花生HIR基因的克隆表達(dá)與進(jìn)化分析[J];山東農(nóng)業(yè)科學(xué);2014年05期

4 馬現(xiàn)永,曹永長(zhǎng),舒鼎銘,畢英佐;肌肉生成抑制因子基因的克隆、表達(dá)及動(dòng)物免疫實(shí)驗(yàn)[J];中國(guó)科學(xué)C輯:生命科學(xué);2004年06期

5 劉文榮;張木清;;抑制性消減雜交技術(shù)在植物基因克隆中的應(yīng)用[J];亞熱帶農(nóng)業(yè)研究;2006年02期

6 史仁玖;殷s，

本文編號(hào)：2310570