【摘要】：為分析基因診斷草莓在干旱逆境條件下生長狀態(tài)的可行性,以‘寧玉’草莓為試材,調(diào)查了不同干旱脅迫條件下的生長特點(diǎn)以及與生長素合成、脫落酸合成、花果發(fā)育、色素合成相關(guān)的10個基因的表達(dá)情況。研究結(jié)果表明:在不同干旱條件下草莓均可完成從營養(yǎng)生長到生殖生長的生命周期,所選基因也都行使并完成了其調(diào)控功能。與對照植株相比,在輕度和中度干旱脅迫(50%~65%土壤持水量)條件下,草莓植株的物候期較對照(80%土壤持水量)提早,而在嚴(yán)重干旱脅迫(35%土壤含水量時)條件下延緩,隨著干旱脅迫條件的加重,草莓植株、葉片、果實(shí)明顯變小,果實(shí)著色加深。每個基因在不同干旱條件下表達(dá)水平的變化趨勢基本一致,且都與相應(yīng)的物候期以及生長發(fā)育時間長短的變化情況相一致,但所選基因在幾種干旱條件下其表達(dá)開始的早晚、表達(dá)水平的高低以及表達(dá)相應(yīng)程度持續(xù)時間的長短存在不同。在輕度和中度干旱條件下,這些基因的表達(dá)時間提早,但持續(xù)時間較短,表達(dá)水平高于對照;而在重度干旱條件下,這些基因的表達(dá)開始的時間較對照延緩,表達(dá)水平較低但持續(xù)時間拉長。上述研究結(jié)果表明,利用基因的表達(dá)信息可以提前推斷草莓植株在不同干旱條件下的生長發(fā)育狀態(tài),了解干旱脅迫對草莓生長發(fā)育的影響以及為是否需要提早采取管理措施等提供重要依據(jù)。
[Abstract]:In order to analyze the feasibility of genetic diagnosis of strawberry growth under drought stress, the growth characteristics of 'Ningyu' strawberry under different drought stress conditions were investigated, and the synthesis of auxin, the synthesis of abscisic acid and the development of flower and fruit were investigated. Expression of 10 genes related to pigment synthesis. The results showed that strawberry could complete the life cycle from vegetative growth to reproductive growth under different drought conditions, and all the selected genes performed their regulatory functions. The phenological period of strawberry plants was earlier than that of control plants under mild and moderate drought stress (50% and 65% soil water holding capacity), but delayed under severe drought stress (35% soil water content). With the increase of drought stress, strawberry plants, leaves and fruits became smaller and the fruit coloration deepened. The trend of expression level of each gene was basically the same under different drought conditions, and was consistent with the change of phenological period and growth and development time, but the expression of selected gene began in the morning and evening under several drought conditions. The level of expression and the duration of the corresponding degree of expression are different. In mild and moderate drought conditions, the expression of these genes was earlier, but the duration was shorter, and the expression level was higher than that of the control, while in severe drought, the time of expression of these genes was slower than that of the control. The expression level is low but the duration is long. The results showed that the growth and development of strawberry plants under different drought conditions could be predicted in advance by using the gene expression information. To understand the effects of drought stress on strawberry growth and development and to provide important basis for early adoption of management measures.
【作者單位】： 南京農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：國家自然科學(xué)基金項(xiàng)目(31401847) 江蘇省自然科學(xué)基金項(xiàng)目(BK20140707) 國家博士后基金項(xiàng)目(2014M561663) 中央高�；究蒲袠I(yè)務(wù)費(fèi)專項(xiàng)資金項(xiàng)目(KJQN201541)
【分類號】：S668.4

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