【摘要】：為探明油菜素內(nèi)酯BR在塊莖萌芽中的作用,建立更有效的種薯催芽調(diào)控體系,選擇了休眠期不同的3個品種,利用q RT-PCR分析與BR合成、信號轉(zhuǎn)導(dǎo)、調(diào)控有關(guān)的9個基因在貯藏期間及抑芽處理下的表達(dá)模式;同時檢測BR類似物24-表油菜素內(nèi)酯(24-e BL)及其與赤霉素GA3對塊莖萌芽的影響。結(jié)果表明,涉及BR合成的4個基因表達(dá)量均隨貯藏時間延長升高,短休眠品種升高的時間點早于中、長休眠品種;信號轉(zhuǎn)導(dǎo)及調(diào)控基因中BRI1和CYCD3的變化與合成基因相似,BSK和TCH4的表達(dá)量則在中、長休眠期品種中保持恒定。抑芽處理在貯藏前期能刺激這些基因的表達(dá)升高,但之后都迅速下降并保持低水平。轉(zhuǎn)錄因子BZR1在各品種中以及抑芽處理下均沒有明顯變化。24-e BL利于塊莖解除休眠,但不促進(jìn)芽的伸長生長,與GA3互配效果更佳,單株塊莖增重37.92%~98.41%。結(jié)論表明,BR合成和信號轉(zhuǎn)導(dǎo)是塊莖從休眠向萌芽轉(zhuǎn)變的必經(jīng)生理過程,它與GA3互配用于催芽更利于種薯萌芽的整齊、健壯并促進(jìn)塊莖形成。
[Abstract]:In order to investigate the role of Brassinolide (BR) in tuber germination, a more effective regulation system of seed tuber germination was established. Three varieties with different dormancy stages were selected. Q RT-PCR analysis, BR synthesis and signal transduction were used. The expression patterns of 9 genes were regulated during storage and bud inhibition, and the effects of 24-e BL (24-e BL), a BR analogue, and gibberellin (GA3) on tuber germination were detected. The results showed that the expression of four genes involved in BR synthesis increased with the prolongation of storage time, and the increase time of short dormancy varieties was earlier than that of medium and long dormancy varieties. The changes of BRI1 and CYCD3 in signal transduction and regulation genes were similar to those in synthetic genes, while the expression of BSK and TCH4 in medium and dormant varieties remained constant. Bud inhibition treatment stimulated the expression of these genes in the early stage of storage, but then decreased rapidly and kept low level. The transcription factor BZR1 did not change significantly in all varieties and under bud inhibition treatment. 24-e BL was beneficial to the release of dormancy from tuber, but did not promote the elongation and growth of buds, and had better effect with GA3. The weight of tuber per plant increased 37.92% and 98.41%. The results showed that BR synthesis and signal transduction were the necessary physiological process of tuber transformation from dormancy to germination, and the combination of GA3 and GA3 was beneficial to the neatness of seed tubers and promote tuber formation.
【作者單位】： 綿陽市農(nóng)業(yè)科學(xué)研究院;四川農(nóng)業(yè)大學(xué)農(nóng)學(xué)院;
【基金】：現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系四川薯類創(chuàng)新團(tuán)隊項目(川農(nóng)業(yè)函[2014]91號) 四川省科技廳公益性育種攻關(guān)項目(2016NYZ0032) 綿陽市農(nóng)業(yè)科學(xué)研究院創(chuàng)新基金項目(cxjj462016-2019)資助~~
【分類號】：S532

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