【摘要】：目的了解貴州省結(jié)核分枝桿菌臨床分離株的數(shù)目可變串聯(lián)重復(fù)序列(MIRU-VNTR)基因多態(tài)性及近期傳播情況。方法收集2014年貴州省結(jié)核分枝桿菌臨床分離株185株(耐多藥菌株57株,全敏感菌株97株),采用MIRU-VNTR 12個(gè)位點(diǎn)分析法進(jìn)行PCR檢測(cè),應(yīng)用NTsys2.10軟件對(duì)全部基因型數(shù)字化結(jié)果進(jìn)行非加權(quán)組平均法(UPGMA)聚類分析。結(jié)果 185株結(jié)核分枝桿菌臨床分離株分為5個(gè)基因群(Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ),呈現(xiàn)156個(gè)基因型,其中135個(gè)為獨(dú)特基因型,其余50株為16簇,最大簇包含10株菌,最小簇包含2株菌,成簇率為27.03%,近期感染最小估計(jì)為18.37%,I群結(jié)核分枝桿菌為147株,占79.46%,為優(yōu)勢(shì)菌群,在MIRU 12個(gè)位點(diǎn)中,26、31位點(diǎn)多態(tài)性較高(h0.60),4、10、23、27、39、40位點(diǎn)呈中等程度的多態(tài)性(0.3≤h≤0.60),2、16、20、24位點(diǎn)多態(tài)性較低(h0.30)。結(jié)論貴州省結(jié)核分枝桿菌具有基因多態(tài)性,其主要的流行群為I群,MIRU26和MIRU31呈高等程度多態(tài)性,MIRU2、MIRU20和MIRU24、MIRU27呈低等程度多態(tài)性。
[Abstract]:Objective to investigate the number of variable tandem repeat (MIRU-VNTR) gene polymorphism and its recent transmission in clinical isolates of Mycobacterium tuberculosis in Guizhou Province. Methods 185 clinical isolates of Mycobacterium tuberculosis (57 multidrug resistant strains and 97 fully sensitive strains) were collected from Guizhou province in 2014. PCR was detected by MIRU-VNTR 12 locus analysis. NTsys2.10 software was used to analyze all the digitized genotypes by unweighted group average method (UPGMA) cluster analysis. Results 185 clinical isolates of Mycobacterium tuberculosis were divided into 5 gene groups (鈪，

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