杜仲橡膠合成相關(guān)酶基因HMGR的克隆與表達(dá)分析
發(fā)布時(shí)間：2018-10-12 11:48
【摘要】：為解析HMGR基因在杜仲橡膠生物合成中的作用,本研究以杜仲良種華仲6號葉片和果皮為材料,參考杜仲基因組和轉(zhuǎn)錄組數(shù)據(jù),克隆了杜仲HMGR基因,命名為Eu HMGR,全長1770 bp,編碼590個(gè)氨基酸。實(shí)時(shí)熒光定量PCR測定結(jié)果顯示,EuHMGR基因在果皮中的表達(dá)量于5月中旬達(dá)到最大值,而在葉片中的最大值出現(xiàn)在7月中旬。通過索氏提取測定相應(yīng)時(shí)期果皮和葉片的含膠量表明,果皮中的含膠量從4月中旬到5月下旬迅速增加,而葉片中的含膠量一直平穩(wěn)增長。分析不同發(fā)育時(shí)期果皮和葉片Eu HMGR基因表達(dá)量和含膠增長速率相關(guān)性發(fā)現(xiàn),果皮Eu HMGR基因在不同發(fā)育時(shí)期的相對表達(dá)量和含膠增長速率呈顯著正相關(guān),而二者在葉片中無明顯相關(guān)性。因此,推測Eu HMGR基因的表達(dá)與杜仲果皮中橡膠生物合成相關(guān)。
[Abstract]:In order to elucidate the role of HMGR gene in the biosynthesis of Eucommia ulmoides HMGR gene was cloned from the leaves and pericarp of Eucommia ulmoides var. ulmoides var.huazhong 6, referring to the genomic and transcriptional data of Eucommia ulmoides. Eu HMGR, was named as full length 1770 bp, encoding 590 amino acids. The results of real-time fluorescence quantitative PCR showed that the expression of EuHMGR gene reached the maximum in the middle of May and the maximum in the leaves in the middle of July. The gel content of pericarp and leaf was measured by Soxhlet extraction. The results showed that the rubber content in pericarp increased rapidly from mid-April to late May, but the content of rubber in leaf increased steadily. By analyzing the correlation between the expression of Eu HMGR gene and the growth rate of gum in pericarp and leaf at different developmental stages, it was found that the relative expression of Eu HMGR gene in pericarp was positively correlated with the growth rate of gum at different developmental stages. However, there was no significant correlation between them in leaves. Therefore, we speculated that the expression of Eu HMGR gene was related to rubber biosynthesis in the pericarp of Eucommia ulmoides.
【作者單位】： 中國林業(yè)科學(xué)研究院經(jīng)濟(jì)林研究開發(fā)中心/國家林業(yè)局杜仲工程技術(shù)研究中心;
【基金】：國家“十二五”科技支撐計(jì)劃(2012BAD21B0502) 國家公益性行業(yè)科研專項(xiàng)(201004029)
【分類號】：S792.99
本文編號：2265995
[Abstract]:In order to elucidate the role of HMGR gene in the biosynthesis of Eucommia ulmoides HMGR gene was cloned from the leaves and pericarp of Eucommia ulmoides var. ulmoides var.huazhong 6, referring to the genomic and transcriptional data of Eucommia ulmoides. Eu HMGR, was named as full length 1770 bp, encoding 590 amino acids. The results of real-time fluorescence quantitative PCR showed that the expression of EuHMGR gene reached the maximum in the middle of May and the maximum in the leaves in the middle of July. The gel content of pericarp and leaf was measured by Soxhlet extraction. The results showed that the rubber content in pericarp increased rapidly from mid-April to late May, but the content of rubber in leaf increased steadily. By analyzing the correlation between the expression of Eu HMGR gene and the growth rate of gum in pericarp and leaf at different developmental stages, it was found that the relative expression of Eu HMGR gene in pericarp was positively correlated with the growth rate of gum at different developmental stages. However, there was no significant correlation between them in leaves. Therefore, we speculated that the expression of Eu HMGR gene was related to rubber biosynthesis in the pericarp of Eucommia ulmoides.
【作者單位】： 中國林業(yè)科學(xué)研究院經(jīng)濟(jì)林研究開發(fā)中心/國家林業(yè)局杜仲工程技術(shù)研究中心;
【基金】：國家“十二五”科技支撐計(jì)劃(2012BAD21B0502) 國家公益性行業(yè)科研專項(xiàng)(201004029)
【分類號】：S792.99
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