【摘要】：為了探究VPS13C和EIF4G1基因與奶山羊產(chǎn)奶性狀的關系,以吐根堡和嶗山奶山羊為試驗組,遼寧絨山羊、日土白絨山羊、班戈白絨山羊和南疆絨山羊為對照組,采用PCR產(chǎn)物直接測序方法,檢測VPS13C基因外顯子10、33和內(nèi)含子10以及EIF4G1基因外顯子18和24上的單核苷酸多態(tài)性在不同山羊品種中的分布。結果表明,在VPS13C基因外顯子10、33和內(nèi)含子10上分別檢測到1、1和3個多態(tài)位點。其中,g.22885CA、g.72809GA位點均導致氨基酸變化。在該基因中發(fā)現(xiàn)2個在奶用和非奶用山羊群體中共有的單體型,分別為ACAG(奶山羊中63.7%;非奶用山羊中21.2%)和CTGT(奶山羊中36.3%;非奶用山羊中78.1%),說明單體型ACAG可能與山羊的產(chǎn)奶性狀相關。在EIF4G1基因外顯子18和24上各發(fā)現(xiàn)1個多態(tài)位點,其中g.12301AG位點導致氨基酸變化,g.9003GA位點不改變氨基酸。g.9003GA位點在嶗山奶山羊上存在AA和AG兩種基因型,與日均產(chǎn)奶量關聯(lián)分析顯示,AA基因型個體的日均產(chǎn)奶量顯著高于AG基因型個體(P=0.021)。研究結果提示:VPS13C和EIF4G1基因均可能是與山羊產(chǎn)奶性狀有一定的相關性,EIF4G1基因中g.9003GA位點與奶山羊高產(chǎn)奶量之間存在顯著相關性。
[Abstract]:In order to explore the relationship between VPS13C and EIF4G1 gene and milk production traits of dairy goats, Tucenburg and Laoshan dairy goats were used as experimental group, Liaoning cashmere goat, Japanese white cashmere goat, Bango white cashmere goat and Nanjiang cashmere goat as control group. The distribution of single nucleotide polymorphisms in exon 1033 and intron 10 of VPS13C gene and exon 18 and 24 of EIF4G1 gene in different goat breeds was detected by direct sequencing of PCR products. The results showed that 1 and 3 polymorphic loci were detected in exon 1033 and intron 10 of VPS13C gene, respectively. Among them, the GA loci of G. 22885 and CAG. 72809 lead to the change of amino acids. Two haplotypes were found in milk and non-milk goat populations, which were ACAG (63.7% in dairy goats; 21.2% in non-dairy goats) and CTGT (36.3% in dairy goats and 78.1% in non-dairy goats), indicating that haplotype ACAG may be related to milk production traits of goats. One polymorphic locus was found on exon 18 and 24 of EIF4G1 gene respectively, in which g.12301AG site led to amino acid change. The amino acid locus. G. 9003GA did not change amino acid. There were two genotypes of AA and AG in Laoshan dairy goat. The correlation analysis with daily milk yield showed that the average daily milk yield of AA genotype individuals was significantly higher than that of AG genotype individuals (P0. 021). The results suggested that the g.9003GA loci in the EIF4G1 gene and the high milk yield of dairy goats were significantly correlated with the g.9003GA gene and the EIF4G1 gene.
【作者單位】： 中國農(nóng)業(yè)科學院北京畜牧獸醫(yī)研究所;黑龍江省農(nóng)業(yè)科學院畜牧研究所;青島農(nóng)業(yè)大學動物科技學院;
【基金】：國家自然基金(31272403,31472064,31601910) 現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術體系(CARS-40-01) 公益性行業(yè)(農(nóng)業(yè))科研專項(201303059) 中央級公益性科研院所基本科研業(yè)務費(2015ywf-zd-1)
【分類號】：S827
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本文編號：2251463