【摘要】：為了獲得藏羊白細(xì)胞介素-7(interleukin-7,IL-7)基因序列,并研究其序列特征及編碼蛋白的結(jié)構(gòu)和功能,本試驗(yàn)采用RT-PCR方法,從藏羊脾臟中擴(kuò)增了IL-7基因,應(yīng)用DNAStar軟件分析該基因的核苷酸和氨基酸序列,與經(jīng)BLAST比對后的參考序列進(jìn)行同源性比對,并構(gòu)建系統(tǒng)進(jìn)化樹,同時(shí)利用DNAStar軟件和在線服務(wù)器預(yù)測該基因編碼蛋白的二級(jí)結(jié)構(gòu)、三級(jí)結(jié)構(gòu)、親水性、信號(hào)肽和蛋白翻譯后修飾位點(diǎn)。結(jié)果表明,藏羊IL-7基因長度為531bp(含終止密碼子),編碼176個(gè)氨基酸。藏羊IL-7基因與綿羊、山羊、藏羚羊、水牛、瘤牛、美洲草原野牛、黃牛和牦牛的IL-7基因核苷酸序列同源性在97.2%~99.8%之間,氨基酸序列同源性在94.9%~99.4%之間,藏羊與綿羊的親緣關(guān)系最近。蛋白結(jié)構(gòu)預(yù)測結(jié)果表明,IL-7蛋白主要由α螺旋組成,是一種親水性和分泌型蛋白。該蛋白含有6種蛋白質(zhì)翻譯后修飾位點(diǎn),包括1個(gè)N-豆蔻�；稽c(diǎn)、1個(gè)酰胺化位點(diǎn)、1個(gè)cAMP和cGMP依賴性蛋白激酶磷酸化位點(diǎn)、3個(gè)N-糖基化位點(diǎn)、4個(gè)蛋白激酶C磷酸化位點(diǎn)、6個(gè)酪蛋白激酶Ⅱ磷酸化位點(diǎn)。本研究結(jié)果可為藏羊IL-7基因的進(jìn)一步研究與臨床應(yīng)用提供參考。
[Abstract]:In order to obtain the sequence of interleukin-7 (interleukin-7,IL-7) gene of Tibetan sheep and study its sequence characteristics and the structure and function of encoded protein, the IL-7 gene was amplified from the spleen of Tibetan sheep by RT-PCR method. The nucleotide and amino acid sequences of the gene were analyzed by DNAStar software, and compared with the reference sequence after BLAST alignment. The phylogenetic tree was constructed. The secondary structure of the gene encoded protein was predicted by DNAStar software and online server. Tertiary structure, hydrophilicity, signal peptide and protein posttranslational modification site. The results showed that the length of IL-7 gene of Tibetan sheep was 531bp, encoding 176 amino acids. The nucleotide sequence homology of IL-7 gene of Tibetan sheep and sheep, goat, Tibetan antelope, buffalo, cattle, buffalo, yellow cattle and yak was 97.2% and 99.8% respectively, and the homology of amino acid sequence between Tibetan sheep and sheep was 99.4%. The relationship between Tibetan sheep and sheep was the most close. The predicted structure of IL-7 protein is mainly composed of 偽 helix and is a hydrophilic and secretory protein. The protein contains six post-translational modification sites. There were one N-nutmeg site, one amidation site, one cAMP and cGMP dependent protein kinase phosphorylation site, three N-glycosylation sites, four protein kinase C phosphorylation sites and six casein kinase 鈪，

本文編號(hào)：2245553