【摘要】：探討齊口裂腹魚(yú)白介素-1β(Interleukin-1β,IL-1β)基因的基本特點(diǎn)。用RT-PCR方法以齊口裂腹魚(yú)脾臟c DNA作為模板擴(kuò)增IL-1β基因,用生物信息學(xué)軟件分析其序列,用RT-PCR方法檢測(cè)IL-1β基因在齊口裂腹魚(yú)6種組織的表達(dá)情況�？寺～@得的IL-1β序列長(zhǎng)1 252 bp,共編碼276個(gè)氨基酸。Gen Bank序列號(hào)為KU886235。IL-1β蛋白相對(duì)分子量為31.25 k D,等電點(diǎn)5.45,預(yù)測(cè)為親水性蛋白。二級(jí)結(jié)構(gòu)主要結(jié)構(gòu)元件為隨機(jī)卷曲和延伸鏈,有4個(gè)N-糖基化位點(diǎn)和16個(gè)磷酸化位點(diǎn)。NJ法系統(tǒng)進(jìn)化樹(shù)顯示,齊口裂腹魚(yú)與鯉魚(yú)、鯽魚(yú)的親緣關(guān)系最近。組織表達(dá)分析顯示,IL-1β基因主要在脾臟和腎臟中表達(dá)。這為深入研究魚(yú)IL-1β的生物學(xué)功能提供理論依據(jù)。
[Abstract]:To study the basic characteristics of interleukin-1 尾 (Interleukin-1 尾) gene in fish. The IL-1 尾 gene was amplified by using c DNA of the spleen of the fish by RT-PCR method. The sequence of IL-1 尾 gene was analyzed by bioinformatics software. The expression of IL-1 尾 gene in 6 kinds of tissues was detected by RT-PCR method. The cloned IL-1 尾 sequence (1 252 bp,) encodes 276 amino acids. Gen Bank sequence number is 31.25 KD, isoelectric point 5.45, predicted to be hydrophilic protein. The main structural elements of the secondary structure were random curl and extension chain. There were 4 N-glycosylation sites and 16 phosphorylation sites. NJ phylogenetic tree showed that the phylogenetic relationship between Carp and Carp, Carassius auratus was the closest. Tissue expression analysis showed that IL-1 尾 gene was mainly expressed in spleen and kidney. This provides a theoretical basis for the further study of the biological function of fish IL-1 尾.
【作者單位】： 西南民族大學(xué)青藏高原研究院;西南民族大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：四川省科技支撐項(xiàng)目(2016NZ0044) 西南民族大學(xué)研究生學(xué)位點(diǎn)建設(shè)項(xiàng)目(2016XWD-SO71007)
【分類(lèi)號(hào)】：S917.4;Q78

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