【摘要】：通過研究赤霉病菌接種后的基因表達(dá),篩選小麥赤霉病抗性相關(guān)基因。根據(jù)前人研究結(jié)果,利用FGENESH軟件對(duì)小麥3B短臂抗赤霉病主效QTL所在的小麥基因組測(cè)序獲得的重疊群ctg0954核苷酸序列進(jìn)行基因預(yù)測(cè),共預(yù)測(cè)得到489個(gè)基因。以攜帶該QTL的蘇麥3號(hào)和未攜帶該QTL的揚(yáng)麥15為材料,在小麥花期進(jìn)行赤霉病菌接種誘導(dǎo)基因表達(dá),接種后0 h、4 h、8 h、12 h、24 h對(duì)其中有功能注釋的259個(gè)預(yù)測(cè)基因的表達(dá)進(jìn)行半定量分析,獲得27個(gè)在品種間差異表達(dá)的基因。這27個(gè)基因的表達(dá)分為4類:一直上調(diào)表達(dá)(Ⅰ型),在接種后的早期上調(diào)表達(dá)后期下調(diào)表達(dá)(Ⅱ型),接種后早期下調(diào)表達(dá)后期上調(diào)表達(dá)(Ⅲ型),接種后一直下調(diào)表達(dá)(Ⅳ型)。在差異表達(dá)基因中,選擇6個(gè)基因,進(jìn)行Real-time PCR分析,結(jié)果表明這些基因在接種病原菌后均上調(diào)表達(dá),且在蘇麥3號(hào)和揚(yáng)麥15號(hào)品種間存在基因表達(dá)差異,驗(yàn)證了半定量表達(dá)結(jié)果,可作為與赤霉病抗性相關(guān)3B短臂主效QTL的候選基因。
[Abstract]:The genes related to scab resistance in wheat were screened by studying the gene expression after inoculation. According to the results of previous studies, 489 genes were predicted by using FGENESH software to predict the nucleotide sequence of overlapped group ctg0954 obtained from wheat genome sequencing of wheat short arm scab resistant to scab. Sumai 3 carrying QTL and Yangmai 15 without QTL were used as materials to induce gene expression by inoculation with scab at flowering stage of wheat. Twenty-seven differentially expressed genes were obtained by semi-quantitative analysis of 259 predictive genes with functional annotation at 0 h, 4 h, 8 h, 12 h and 24 h after inoculation. The expression of these 27 genes can be classified into four categories: up-regulated expression (type 鈪，

本文編號(hào)：2214673