基于HRM體系開發(fā)抗稻瘟病基因Pi2特異性分子標記
發(fā)布時間:2018-08-29 14:08
【摘要】:Pi2是一個具有廣譜抗性的稻瘟病抗性基因,利用分子標記輔助選擇技術選育含有Pi2抗性基因的水稻品種已得到越來越廣泛的應用。本研究根據(jù)稻瘟病抗性基因Pi2及其復等位基因的序列,利用HRM技術開發(fā)與Pi2完全共分離的分子標記,并結合水稻DNA快速提取方法,建立中高通量輔助選擇體系。該方法體系準確高效、簡便快速、成本低廉,能夠大大提高抗病育種選育的效率,為分子標記輔助選擇育種或者水稻種質資源Pi2基因型鑒定提供技術選擇。
[Abstract]:Pi2 is a broad-spectrum resistant rice blast resistance gene. The selection of rice varieties with Pi2 resistance gene by molecular marker-assisted selection technology has been more and more widely used. Based on the sequence of rice blast resistance gene Pi2 and its complex alleles, a molecular marker completely separated from Pi2 was developed by using HRM technique, and a medium and high throughput assisted selection system was established by combining with the rapid extraction method of DNA in rice (Oryza sativa L.). The method is accurate, efficient, simple, rapid and low cost. It can greatly improve the efficiency of breeding for disease resistance, and provide a technical choice for molecular marker-assisted selection or Pi2 genotyping of rice germplasm resources.
【作者單位】: 華南農(nóng)業(yè)大學生命科學學院;深圳市作物分子設計育種研究院深圳市分子設計育種重點實驗室;
【基金】:國家自然科學基金青年基金項目(31500254) 廣東省引進創(chuàng)新科研團隊計劃資助項目(No.201001S010472-5509) 深圳市孔雀計劃引進團隊計劃資助項目(KQF201109160004A) 深圳市技術攻關項目(JSGG20150508105340526) 深圳市農(nóng)業(yè)技術成果轉化項目(NYKJ20140922151049440)共同資助
【分類號】:S435.111.41
本文編號:2211435
[Abstract]:Pi2 is a broad-spectrum resistant rice blast resistance gene. The selection of rice varieties with Pi2 resistance gene by molecular marker-assisted selection technology has been more and more widely used. Based on the sequence of rice blast resistance gene Pi2 and its complex alleles, a molecular marker completely separated from Pi2 was developed by using HRM technique, and a medium and high throughput assisted selection system was established by combining with the rapid extraction method of DNA in rice (Oryza sativa L.). The method is accurate, efficient, simple, rapid and low cost. It can greatly improve the efficiency of breeding for disease resistance, and provide a technical choice for molecular marker-assisted selection or Pi2 genotyping of rice germplasm resources.
【作者單位】: 華南農(nóng)業(yè)大學生命科學學院;深圳市作物分子設計育種研究院深圳市分子設計育種重點實驗室;
【基金】:國家自然科學基金青年基金項目(31500254) 廣東省引進創(chuàng)新科研團隊計劃資助項目(No.201001S010472-5509) 深圳市孔雀計劃引進團隊計劃資助項目(KQF201109160004A) 深圳市技術攻關項目(JSGG20150508105340526) 深圳市農(nóng)業(yè)技術成果轉化項目(NYKJ20140922151049440)共同資助
【分類號】:S435.111.41
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