【摘要】：通過對尾突臂尾輪蟲、裂足臂尾輪蟲、剪形臂尾輪蟲、方形臂尾輪蟲、紅臂尾輪蟲、鐮形臂尾輪蟲、矩形臂尾輪蟲和十指臂尾輪蟲8種臂尾輪蟲的ND4和ND5基因序列進(jìn)行擴(kuò)增、測序,結(jié)合Gen Bank數(shù)據(jù)庫中的褶皺臂尾輪蟲、萼花臂尾輪蟲、轉(zhuǎn)輪蟲和旋輪蟲的ND4和ND5基因序列,使用MEGA6.0軟件構(gòu)建這12種輪蟲系統(tǒng)發(fā)生樹(NJ樹、ME樹、UPGMA樹),探討了8種臂尾輪蟲之間的系統(tǒng)關(guān)系。結(jié)果顯示,本研究所涉及的輪蟲ND4和ND5基因序列差異百分比均值為40.3%,可作為分子標(biāo)記應(yīng)用于輪蟲屬內(nèi)種間系統(tǒng)關(guān)系研究;系統(tǒng)樹所顯示親緣關(guān)系與形態(tài)學(xué)研究基本一致,均支持將十指臂尾輪蟲、裂足臂尾輪蟲歸入臂尾輪屬。
[Abstract]:The ND4 and ND5 gene sequences of 8 species of rotifer, and rotifer were amplified. The ND4 and ND5 genes of Brachionus pleuroides, Rotifera calyx, rotifer and rotifer were sequenced and sequenced in the Gen Bank database, and the ND4 and ND5 gene sequences of rotifer, rotifer and rotifer were sequenced. The phylogenetic tree of 12 species of rotifers (NJ tree ME tree UPGMA tree) was constructed by using MEGA6.0 software. The systematic relationship between 8 species of rotifers was discussed. The results showed that the average percentage difference between ND4 and ND5 genes was 40.3, which could be used as a molecular marker to study the interspecific phylogenetic relationship of rotifers, and the phylogenetic relationship showed by the phylogenetic tree was basically consistent with the morphological study. All of them support the division of the rotifer and the rotifer into the genus Brachiodendron.
【作者單位】： 安徽師范大學(xué)生命科學(xué)學(xué)院;安徽省高校生物環(huán)境與生態(tài)安全省級重點(diǎn)實驗室;安徽省重要生物資源保護(hù)與利用研究省級重點(diǎn)實驗室;
【基金】：國家自然科學(xué)基金(31470015) 安徽省自然科學(xué)基金(1408085MC66)~~
【分類號】：Q959.181
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本文編號：2205080