【摘要】：目的:探索一種對(duì)人體無害、無痛、操作簡(jiǎn)便的獲取基因組脫氧核糖核酸(deoxyribonucleic acid,DNA)的方法。方法:從唾液中提取基因組DNA,應(yīng)用聚合酶鏈?zhǔn)椒磻?yīng)(polymerase chain reaction,PCR)擴(kuò)增血紅蛋白β基因,采用含溴乙錠的瓊脂糖凝膠電泳鑒定擴(kuò)增產(chǎn)物。結(jié)果:從唾液中提取基因組DNA能夠擴(kuò)增出血紅蛋白β基因。結(jié)論:唾液作為獲取基因組脫氧核糖核酸的簡(jiǎn)易來源,取樣方法簡(jiǎn)便、快捷,更適用于疾病研究中大規(guī)模群體篩查。
[Abstract]:Objective: to explore a simple and convenient method for obtaining genomic deoxyribonucleic acid (deoxyribonucleic acido DNA). Methods: genomic DNA was extracted from saliva. Hemoglobin 尾 gene was amplified by polymerase chain reaction (PCR) and agarose gel electrophoresis with ethidium bromide. Results: hemoglobin 尾 gene could be amplified by extracting genomic DNA from saliva. Conclusion: as a simple source of genomic DNA, saliva is a simple and rapid sampling method, which is more suitable for mass screening in disease research.
【作者單位】： 包頭醫(yī)學(xué)院生物化學(xué)教研室;包頭醫(yī)學(xué)院基因診斷研究所;包頭醫(yī)學(xué)院血紅蛋白研究室;
【基金】：包頭市科技計(jì)劃項(xiàng)目(2015S2004-8) 包頭醫(yī)學(xué)院博士科研啟動(dòng)基金項(xiàng)目(BSJJ201605)
【分類號(hào)】：R440

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