【摘要】：由大豆疫霉引起的大豆疫霉根腐病是一種世界性大豆病害,對產(chǎn)量品質(zhì)影響極大。利用抗病品種進(jìn)行防治是目前最經(jīng)濟(jì)、有效的方法。大豆對疫霉菌株的抗性分為由單基因控制的完全抗性和由多基因控制的部分抗性兩種。以對多個大豆疫霉生理小種具有抗性的大豆品種鄭97196作為抗性親本與大豆感病品系X242003雜交構(gòu)建F_(2∶3)重組自交家系群體,用大豆疫霉菌株He N35對親本及F_(2∶3)群體進(jìn)行抗性遺傳分析并利用SSR技術(shù)對鄭97196的抗性基因進(jìn)行定位。結(jié)果表明:鄭97196對大豆疫霉抗性是由一對顯性單基因控制的,抗病對感病表現(xiàn)為顯性,再用9種毒力公式不同的疫霉菌株分別接種鄭97196和14個國內(nèi)外公認(rèn)的含有單個抗病基因的大豆品種(鑒別寄主),觀察并記錄抗性反應(yīng)類型,結(jié)果顯示鄭97196的抗性反應(yīng)類型與14個鑒別寄主有所不同,推測其可能含有新的抗病基因,暫命名為Rps Zheng。通過SSR分子作圖分析,該基因位于大豆分子遺傳圖譜N連鎖群上satt485和satt584之間,與這兩個標(biāo)記之間的距離分別為2.1和3.7cM。
[Abstract]:Phytophthora sojae root rot caused by Phytophthora sojae is a worldwide disease of soybean, which has a great impact on Yield and quality. It is the most economical and effective method to control the disease-resistant varieties. Resistance of soybean to Phytophthora sojae can be divided into complete resistance controlled by single gene and partial resistance controlled by multiple genes. Zheng 97196, a soybean variety with resistance to Phytophthora sojae, was crossed with soybean susceptible strain X242003 to construct F_ (2:3) recombinant inbred family population. The resistance genetic analysis of the parents and F_ (2:3) population of Phytophthora sojae strain He N35 was carried out and the resistance gene of Zheng 97196 was mapped by SSR technique. Resistance to Phytophthora sojae was controlled by a pair of dominant single genes. Resistance to Phytophthora sojae was dominant. Nine strains of Phytophthora sojae with different virulence formulas were inoculated with Zheng 97196 and 14 soybean cultivars (identification hosts) with a single resistance gene recognized at home and abroad. The resistance types were observed and recorded. The results showed that Zheng 97196 was resistant to Phytophthora so The reaction type was different from that of the 14 identified hosts. It was speculated that the gene might contain a new resistance gene, tentatively named Rps Zheng. SSR molecular mapping analysis showed that the gene was located between satt485 and satt584 in the N linkage group of soybean molecular genetic map, and the distances between the two markers were 2.1 and 3.7 cM, respectively.
【作者單位】： 南京農(nóng)業(yè)大學(xué)大豆研究所/國家大豆改良中心/農(nóng)業(yè)部大豆生物學(xué)與遺傳育種重點實驗室(綜合)/作物遺傳與種質(zhì)創(chuàng)新國家重點實驗室;
【基金】：國家公益性行業(yè)(農(nóng)業(yè))科研專項經(jīng)費(201303018) 優(yōu)質(zhì)高產(chǎn)高油大豆種質(zhì)創(chuàng)新與新品種選育(BE2013350) 江蘇省自然科學(xué)基金項目(BK2011637和BK20130684) 國家現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系(CARS-04-PS10) 江蘇省現(xiàn)代作物生產(chǎn)協(xié)同創(chuàng)新中心
【分類號】：S435.651

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 杜永;潘啟民;徐大勇;徐敏權(quán);;水稻白化苗性狀的遺傳分析[J];植物遺傳資源科學(xué);2002年01期

2 李偉;智慧;王永芳;李海權(quán);刁現(xiàn)民;;谷子類病斑突變體09-1115的發(fā)現(xiàn)及遺傳分析[J];河北農(nóng)業(yè)科學(xué);2010年11期

3 王克榮,丁國云;栗疫病菌表型特征的遺傳分析[J];植物病理學(xué)報;1998年02期

4 羅寬,黃聲儀,王國平,萬文舉;珍龍13和窄葉青8號抗稻瘟病遺傳分析[J];植物病理學(xué)報;1985年02期

5 姚占軍,徐世昌,吳立人;小麥抗病性遺傳的研究進(jìn)展[J];遼寧農(nóng)業(yè)科學(xué);2002年06期

6 譚琦,C.PeterRomaine,BethSchlagnhaufer;利用RAPD技術(shù)對不同地區(qū)的有害疣孢霉菌株進(jìn)行遺傳分析[J];食用菌學(xué)報;1996年04期

7 姚方印,朱常香,李廣賢,溫孚江;Bt水稻的抗蟲性鑒定及轉(zhuǎn)基因的遺傳分析[J];中國農(nóng)業(yè)科學(xué);2002年02期

8 蔣冬花,郭澤建;轉(zhuǎn)隱地蛋白基因煙草的抗病性及遺傳分析[J];植物病理學(xué)報;2003年03期

9 劉勝毅,周必文,張建坤,郭學(xué)蘭,許澤永,何禮遠(yuǎn);甘藍(lán)型油菜抗菌核病擴(kuò)展的遺傳分析[J];中國油料作物學(xué)報;2003年01期

10 莊勇;王述彬;;茄子近緣種黃萎病抗性鑒定及遺傳分析[J];江蘇農(nóng)業(yè)學(xué)報;2009年04期

相關(guān)會議論文 前1條

1 崔鈺;Naxin Huo;John P.Vogel;David F.Garvin;Andrew O.Jackson;劉志勇;;對二穗短柄草中抗大麥條紋花葉病毒基因的遺傳分析[A];中國植物病理學(xué)會2011年學(xué)術(shù)年會論文集[C];2011年

相關(guān)博士學(xué)位論文 前1條

1 陳時盛;小麥抗銹病基因的遺傳分析及其分子作圖[D];四川農(nóng)業(yè)大學(xué);2015年

，

本文編號：2194956