本文關(guān)鍵詞：轉(zhuǎn)基因水稻T-DNA側(cè)翼序列的擴(kuò)增與分析，由筆耕文化傳播整理發(fā)布。

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側(cè)翼

    Cloning and Characterization of the 5' Flanking Sequences of CD2 Gene

    人淋巴細(xì)胞CD2基因5’側(cè)翼序列的克隆和鑒定

短句來(lái)源

    TAIL-PCR technique was performed to get the flanking sequence of T-DNA tags(FSTs) from six randomly selected HDF-68 positive T-DNA transformants only in one day time. After analysis of its sequences,the main factors of influencing TAIL-PCR amplification were discussed.

    采用TAIL-PCR技術(shù),對(duì)隨機(jī)選取的6個(gè)HDF-68陽(yáng)性T-DNA轉(zhuǎn)化子進(jìn)行擴(kuò)增,只利用1 d的時(shí)間,即獲取了T-DNA標(biāo)簽側(cè)翼序列(FSTs),對(duì)其序列進(jìn)行分析后,分析了影響TAIL-PCR擴(kuò)增的主要因素.

短句來(lái)源

    ERIC(IRU) probably modulates the expression of flanking genes.

    ERIC(IRU)很可能調(diào)節(jié)側(cè)翼基因(flanking gene)的表達(dá)。

短句來(lái)源

    Analysis of the Mouse Nodal Gene Promoter on Its 5' Flanking Sequence

    Nodal基因5′末端側(cè)翼序列啟動(dòng)子的分析

短句來(lái)源

    Analysis of an Inhancer Element of Mouse Nodal Gene in its 5' Flanking Sequence

    Nodal基因5'末端側(cè)翼序列增強(qiáng)子的分析

短句來(lái)源

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    Cloning and Characterization of the 5' Flanking Sequences of CD2 Gene

    人淋巴細(xì)胞CD2基因5’側(cè)翼序列的克隆和鑒定

短句來(lái)源

    Amplification and Analysis of T-DNA Flanking Sequences in Transgenic Rice

    轉(zhuǎn)基因水稻T-DNA側(cè)翼序列的擴(kuò)增與分析

短句來(lái)源

    Selection of Optimal Primers for TAIL-PCR in Identifying Ds Flanking Sequences from Ac/Ds Insertion Rice Lines

    從水稻Ac/Ds插入突變體擴(kuò)增Ds側(cè)翼序列的最適TAIL-PCR引物(英文)

短句來(lái)源

    Analysis ofcbf1Flanking Sequences in Transgenic Tobacco by TAIL-PCR

    用改良TAIL-PCR技術(shù)分析轉(zhuǎn)基因煙草cbf1基因插入?yún)^(qū)的側(cè)翼序列

短句來(lái)源

    Amplification of Ds Flanking Sequences from Rice Genomic DNA of Hybrids of Ac×Ds Lines and the Analysis of Ds Insertions

    水稻Ac×Ds后代基因組DNA中Ds側(cè)翼序列的擴(kuò)增及其Ds插入分析

短句來(lái)源

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    1. For the purpose of constructing mammary gland specific expressional vector, the bovine 3 -lactoglobulin gene 5' flanking fragment was cloned by PCR amplification. It consisted of part of the gene upstream region about 645bp> the first exon and the first intron about 804bp.

    1．利用PCR的方法克隆了牛β-乳球蛋白基因5′調(diào)控成分(1449bp)，其中包括5′側(cè)翼序列(645bp)，第一外顯子及第一內(nèi)含子(804bp)。

短句來(lái)源

    2. The ATG which was the translation start code of bovine f3 -lactoglobulin gene 5' flanking fragment consisting in part of the gene upstream region about 645bp, the first exon and the first intron about 804bp was mutated to AAG by PCR amplification. The mutant fragment was marked as MBLG fragment.

    2．利用PCR方法將牛β-乳球蛋白基因5′調(diào)控成分(長(zhǎng)度為1449bp，包括645bp的5′側(cè)翼序列，，804bp的第一外顯子及第一內(nèi)含子序列)中的翻譯起始密碼子ATG突變?yōu)锳AG，獲得MBLG片段。

短句來(lái)源

    For the purpose of constructing mammary gland specific expressional vector,the bovine β lactoglobulin gene 5′ flanking fragment was cloned by PCR amplification. It consisted in part of the gene upstream region about 645 bp,the first exon and the first intron about 804 bp.

    利用 PCR方法克隆了牛 β-乳球蛋白基因 5′調(diào)控成分 (1 4 4 9bp) ,其中包括 5′側(cè)翼序列 (6 4 5 bp) ,第一外顯子及第一內(nèi)含子 (80 4 bp)。

短句來(lái)源

    A recombinant plasmid pGL3-Basic-5.5K containing the approximate 5.5K region around the translation initiation codon was constructed.

    有多個(gè)潛在的啟動(dòng)子區(qū)段。 成功構(gòu)建了重組質(zhì)粒pGL3-Basic-5.5K,其插入片斷包含人BMPR2基因5′側(cè)翼近5.5 K的序列。

短句來(lái)源

    TAIL-PCR:A Simple and Efficient Method to Isolate DNA Segments Adjacent to Known Sequence

    一種DNA側(cè)翼序列分離技術(shù)——TAIL-PCR

短句來(lái)源

    Sequence and structure analysis of mitochondrial D-loop and tRNA~(thr) & tRNA~(pro) genes in Xiphophorus helleri

    劍尾魚線粒體DNA D-環(huán)及側(cè)翼tRNA~(thr)和tRNA~(pro)序列與結(jié)構(gòu)分析

短句來(lái)源

    Mechanism of Transcriptional Activation of LRP16 Gene Expression by 17-Estradiol via 5′-Flanking GC-rich Region

    雌激素通過(guò)LRP16基因5′側(cè)翼GC富含區(qū)上調(diào)其轉(zhuǎn)錄的分子機(jī)制

短句來(lái)源

    Cloning and analyzing the 5′region of follicle stimulating hormone receptor(FSHR) gene on buffalo

    水牛促卵泡素受體(FSHR)基因5′端側(cè)翼序列的克隆與分析

短句來(lái)源

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  flanking

Consensus Sequence of Transcription Factor SF-1 Binding Site and Putative Binding Site in the 5'-Flanking Regions of Genes Encod

5'-Flanking regions of genes encoding Cyp17 and 3βHSDI were shown to contain six and five such binding sites, respectively.

The dependence of exonuclease activity on the "canonicity" of the base pair formed by dNMP flanking the nick at the 3' end, on the nature of the group flanking the nick at the 5' end, and on the reaction conditions has been determined.

The misperception of the stimulus size was dependent on the subject's age, the distance between the circles, and the size of the flanking circles.

The smaller flanking circles located at any distance produced size underestimation in younger children; these circles led to size overestimation in case of their location at a short distance in most adults and adolescents.

更多          

  flanking sequences

No homology was observed between operon-flanking sequences of the two closely related species, suggesting horizontal transfer of the operon.

In particular, L1 retrotransposons, which occupy approximately 20% of genomic DNA, transduce their 3'-flanking sequences to new genomic loci and create pseudogenes via reverse transcription of different types of cellular RNAs.

Several elements in the 5'-flanking (e.g., CAAT and TATA box) and the 3'-flanking sequences have been detected, which has been shown to be necessary for the proper expression of gliadins.

Protein splicing is a posttranslational process that results in excision of an internal protein region (intein) and ligation of its flanking sequences (exteins).

The same was true for its central 2.4-kb fragment devoid of flanking sequences.

更多          

  flanking fragment

PMA-inducible promotor activity was contained in a 700 base pair 5' flanking fragment conserved with the mouse MAdCAM-1 gene including tandem NF-kB sites, and an Sp1 site; and in addition multiple potential AP2, Adh1 (ETF), PEA3, and Sp1 sites.

Reporter assays of catfish B cells transfected with a 742-bp VL-flanking fragment showed promoter activity in the absence of enhancer elements.

A 647-bp 5'-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene.

By screening a genomic library of maize, a 2.2?kb 5' flanking fragment of Zpu1 gene, encoding the pullulanase-type starch debranching enzyme, was isolated.

The 5' flanking fragment of the isolated gene was previously shown to direct endosperm-specific expression of a reporter gene in transgenic tobacco.

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One species of Oesophagostomum from the swine is described and considered as new to science. Oesopkagostomum hsiungi n. sp. may be distinguished from the other species belonging to the same genus by having no cervical groove, no cephalic vesieal and no lateral cervical alae

本文報(bào)導(dǎo)了豬的結(jié)節(jié)蟲一新種，定名為熊氏結(jié)節(jié)蟲。這種結(jié)節(jié)蟲缺頭泡、頭溝和側(cè)翼膜，因之很易區(qū)別于他種結(jié)節(jié)蟲。最后，作者建議將熊大仕、孔繁瑤兩氏（1955）所擬的家畜結(jié)節(jié)蟲檢索表稍加補(bǔ)充，以使新種結(jié)節(jié)蟲也得以包括在內(nèi)。

The electromyography of the superior and inferior heads of lateral pterygoid muscle of 15 human subjects were recorded. It has been observed that marked active potential occurred in the superior head of the ipsilateral pterygoid muscle and the inferior head of the contralateral muscle during a one-side molar bite. Marked active Potential appeared in the superior head of the lateral pterygoid muscle during the closing movement of the jaw, whereas it appeared in the inferior head during the opening movement.

在15例健康人體上,用兩支同芯針電極插入翼外肌,同時(shí)記錄翼外肌上頭及下頭的動(dòng)作電位。肌電圖顯示咬一側(cè)磨牙時(shí),同側(cè)的翼外肌上頭有明顯動(dòng)作電位,對(duì)側(cè)翼外肌下頭的動(dòng)作電位較上頭明顯。張口時(shí)翼外肌下頭有明顯的動(dòng)作電位,閉口時(shí)外肌上頭有明顯的動(dòng)作電位。下頜骨前伸時(shí)翼外肌上頭及下頭只有輕度動(dòng)作電位,未能證實(shí)傳統(tǒng)教科書所說(shuō)翼外肌為一重要的下頒骨前伸肌的描述。下頜骨后退時(shí),翼外肌上頭有極輕度動(dòng)作電位。下頜骨作側(cè)向運(yùn)動(dòng)時(shí),同側(cè)翼外肌的動(dòng)作電位明顯,對(duì)側(cè)翼外肌上、下頭只有輕度動(dòng)作電位,未能證實(shí)科書所說(shuō)單側(cè)翼外肌收縮,使下頷骨向?qū)?cè)運(yùn)動(dòng)的描述。從肌電記錄及形態(tài)結(jié)構(gòu)觀察,翼外肌上頭及下頭是功能上及結(jié)構(gòu)上都不相同的兩塊肌,應(yīng)有所區(qū)別。

Distribution of LH cells in the anterior pituitary of the female rats were studied using the horseradish peroxidase immunohistochemical method. It was shown that LH cells arerelatively concentrated in the following regions of the pars distalis: a) the ventral regionof cephalic pars. b) the dorsal region of cephaic pars and the bilateral wings near theintermedial lobe. c) the dorsal region. of the caudal part. The characteristic distributionof LH cells was clearer in ovariectomized rats. We presumed that the...

Distribution of LH cells in the anterior pituitary of the female rats were studied using the horseradish peroxidase immunohistochemical method. It was shown that LH cells arerelatively concentrated in the following regions of the pars distalis: a) the ventral regionof cephalic pars. b) the dorsal region of cephaic pars and the bilateral wings near theintermedial lobe. c) the dorsal region. of the caudal part. The characteristic distributionof LH cells was clearer in ovariectomized rats. We presumed that the distribution of theLH cell in the anterior pituitary, to a certain extent, might be related to portal vessels.

應(yīng)用辣根過(guò)氧化物酶標(biāo)記抗體技術(shù),對(duì)正常和閹割成年雌性大白鼠垂體LH細(xì)胞的分布進(jìn)行了研究。結(jié)果發(fā)現(xiàn)在大白鼠垂體遠(yuǎn)側(cè)部以下三個(gè)區(qū)域的LH細(xì)胞數(shù)量多、細(xì)胞大、分布集中:(1)頭端部的腹部區(qū);(2)頭端部的背部區(qū)和鄰近中間葉的兩側(cè)翼;(3)尾端部的背部區(qū)和鄰近中間葉的兩側(cè)翼。閹割大白鼠在這三個(gè)區(qū)域內(nèi)分布集中的特點(diǎn)比正常大白鼠更明顯。作者認(rèn)為L(zhǎng)H細(xì)胞這種有規(guī)律的分布與垂體門脈血管的分布有依賴關(guān)系,并就此結(jié)果進(jìn)行了討論。

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  本文關(guān)鍵詞：轉(zhuǎn)基因水稻T-DNA側(cè)翼序列的擴(kuò)增與分析，由筆耕文化傳播整理發(fā)布。