【摘要】：為評價H9N2亞型禽流感病毒(AIV)NS1基因缺失減毒活疫苗候選株r TX-NS1-128對蛋雞的免疫效力,本研究對蛋雞H9N2亞型AIV母源抗體半衰期、疫苗適宜免疫時間、抗體免疫保護臨界值以及免疫雞抗體消長規(guī)律等進行研究。結(jié)果顯示,雛雞H9亞型AIV母源血凝抑制(HI)抗體半衰期為3.29 d。雛雞母源抗體水平對疫苗的免疫效果有較大的影響,以母源HI抗體滴度約為4 log2時免疫為宜。少數(shù)HI抗體滴度為4 log2~6 log2的免疫雞攻毒后可在5 d或3 d內(nèi)從咽喉短暫排毒,HI抗體滴度為4 log2、5 log2、6 log2和≥7 log2時攻毒保護率分別為80%、90%、90%和100%,建議該疫苗的抗體免疫保護臨界值為5 log2。雞一次免疫后平均HI抗體滴度在5 log2以上可持續(xù)9周左右。本研究為H9N2亞型禽流感新型減毒活疫苗的開發(fā)和應用提供依據(jù)。
[Abstract]:In order to evaluate the immune efficacy of live attenuated vaccine candidate r TX-NS1-128 for (AIV) NS1 gene deletion of H9N2 subtype avian influenza virus (Avian Influenza virus) in laying hens, the half-life of maternal antibody against AIV subtype of H9N2 subtype of laying hens and the appropriate immunization time of the vaccine were studied in this study. The critical value of antibody immune protection and the rule of antibody growth and decline in immunized chicken were studied. The results showed that the half life of hemoagglutination inhibiting (HI) antibody of chicken H9 subtype AIV was 3.29 days. The maternal antibody level of chicks had a great influence on the immune effect of the vaccine, and the best immunization was when the titer of maternal HI antibody was about 4 log2. A few immunized chickens with HI antibody titer of 4 log2~6 log2 could detoxify HI antibody from pharynx and larynx within 5 days or 3 days after inoculation. The protective rates of HI antibody from larynx and throat were 80% and 100% respectively when HI titer was 4 log2 and 5 log2 and 鈮，

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