發(fā)布時(shí)間：2018-08-05 20:28

【摘要】：從‘章姬’草莓中克隆了一個(gè)MOT1家族成員的全長(zhǎng),測(cè)序發(fā)現(xiàn)其CDS序列與公布的森林草莓基因組序列一致,將其命名為FaMOT1。進(jìn)化樹分析表明Fa MOT1所編碼的氨基酸與蘋果和桃親緣關(guān)系最近。熒光定量PCR結(jié)果表明,FaMOT1基因在草莓的根、莖、葉、花和10 d幼果中有不同程度的表達(dá)。對(duì)草莓幼苗噴施不同濃度的鉬酸鈉,結(jié)果發(fā)現(xiàn),各處理葉片的鉬濃度隨著施鉬量的增加而顯著提高。2.4 mmol·L~(-1) Na2Mo O4處理顯著誘導(dǎo)根部和葉片的FaMOT1的表達(dá),處理2和4 h時(shí)4.8 mmol·L~(-1) Na_2MoO_4處理的莖部FaMOT1表達(dá)水平最高,處理6和12 h時(shí)2.4 mmol·L~(-1) Na_2MoO_4處理的莖部FaMOT1表達(dá)水平最高。2.4 mmol·L~(-1) Na_2MoO_4處理葉片的硝態(tài)氮濃度顯著低于其它處理,氨態(tài)氮濃度顯著高于其它處理;4.8 mmol·L~(-1)Na_2MoO_4處理根部和葉片的氨態(tài)氮濃度最低。以上結(jié)果表明FaMOT1基因的表達(dá)受到鉬的誘導(dǎo)和調(diào)節(jié),合適濃度的鉬處理有利于硝態(tài)氮的吸收和向氨態(tài)氮的轉(zhuǎn)化以及氨態(tài)氮向有機(jī)氮的轉(zhuǎn)化。
[Abstract]:A member of MOT1 family was cloned from 'Zhang Ji' strawberry, and its CDS sequence was found to be consistent with the published genomic sequence of forest strawberry, which was named FaMOT 1. Phylogenetic tree analysis showed that the amino acids encoded by Fa MOT1 were most closely related to apple and peach. The results of fluorescence quantitative PCR showed that Fam MOT1 gene was expressed in different degree in roots, stems, leaves, flowers and 10-day-old fruits of strawberry. Different concentrations of sodium molybdate were sprayed on strawberry seedlings. The results showed that the molybdenum concentration in leaves increased significantly with the increase of molybdenum application. The FaMOT1 expression in roots and leaves was significantly induced by the treatment of .2.4 mmol L-1 Na2Mo O4. The highest level of FaMOT1 expression was observed in stems treated with 4.8 mmol L ~ (-1) Na_2MoO_4 for 2 h and 4 h, and the highest FaMOT1 expression level in stems treated with 2.4 mmol L ~ (-1) Na_2MoO_4 at 6 h and 12 h. The nitrate N concentration of leaves treated with 2.4 mmol L ~ (-1) Na_2MoO_4 was significantly lower than that of other treatments. The concentration of ammonia nitrogen was significantly higher than that of other treatments (4.8 mmol L ~ (-1) Na_2MoO_4). The lowest concentration of ammonia nitrogen was found in roots and leaves. The results indicated that the expression of FaMOT1 gene was induced and regulated by molybdenum, and the proper concentration of molybdenum was beneficial to the absorption of nitrate nitrogen, the transformation to ammonia nitrogen and the transformation of ammonia nitrogen to organic nitrogen.
【作者單位】： 作物生物學(xué)國(guó)家重點(diǎn)實(shí)驗(yàn)室山東果蔬優(yōu)質(zhì)高效生產(chǎn)協(xié)同創(chuàng)新中心山東農(nóng)業(yè)大學(xué)園藝科學(xué)與工程學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31672137) 山東省現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系果品創(chuàng)新團(tuán)隊(duì)-栽培與土肥崗(SDAIT-06-01)~~
【分類號(hào)】：S668.4
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本文編號(hào)：2166926