【摘要】：堿性亮氨酸拉鏈(bZIP)轉(zhuǎn)錄因子是真核生物轉(zhuǎn)錄因子中分布最廣泛、最保守的一類蛋白。目前在許多植物中已發(fā)現(xiàn)大量的bZIP轉(zhuǎn)錄因子,這些bZIP轉(zhuǎn)錄因子成員廣泛參與種子貯藏基因的表達、植物的生長發(fā)育、光信號傳導(dǎo)、病害防御、生物和非生物脅迫應(yīng)答以及ABA的敏感性等各種信號的反應(yīng)。本研究首次從紫花苜蓿(Medicago sativa)全轉(zhuǎn)錄組水平鑒定出bZIP轉(zhuǎn)錄因子家族共包含138個基因,根據(jù)bZIP蛋白序列進行系統(tǒng)進化分析可以將其分為10類;對MsbZIP基因的系統(tǒng)進化分析表明該基因家族在分類上有很高的保守性。該轉(zhuǎn)錄因子家族的基因密碼子偏好性分析表明,MsbZIP基因密碼子偏好使用A/T堿基。此外,MsbZIP基因GO功能注釋分析結(jié)果顯示,138個MsbZIP基因最終分為23個GO分類,總體包括分子功能和生物學(xué)過程兩類。相關(guān)性分析結(jié)果表明,共有372對基因表達相關(guān)性極顯著(P0.01)。本研究可為紫花苜蓿bZIP轉(zhuǎn)錄因子功能特性、進化歷程和生物功能的深入研究奠定基礎(chǔ)。
[Abstract]:Basic leucine zipper (bZIP) transcription factor is the most widely distributed and conserved class of eukaryotic transcription factors. At present, a large number of bZIP transcription factors have been found in many plants. These bZIP transcription factors are widely involved in the expression of seed storage genes, plant growth and development, light signal transduction, disease defense. Biological and abiotic stress response and ABA sensitivity and other signal responses. In this study, the bZIP transcription factor family was identified for the first time from the (Medicago sativa) full transcriptome level of alfalfa. The bZIP transcription factor family contains 138 genes, which can be classified into 10 categories by phylogenetic analysis of bZIP protein sequences. Phylogenetic analysis of MsbZIP gene showed that the gene family was highly conserved in classification. The codon preference analysis of the transcription factor family showed that the codon of MsbZIP gene preferred to use the A / T base. In addition, the results of go functional annotation analysis of MsbZIP gene showed that 138 MsbZIP genes were classified into 23 go categories, including molecular function and biological process. The results of correlation analysis showed that 372 pairs of genes were significantly correlated (P0.01). This study can lay a foundation for the further study of the functional characteristics, evolutionary process and biological functions of bZIP transcription factors in alfalfa.
【作者單位】： 中國農(nóng)業(yè)科學(xué)院草原研究所;全國畜牧總站全國草品種審定委員會辦公室;草地農(nóng)業(yè)生態(tài)系統(tǒng)國家重點實驗室蘭州大學(xué)草地農(nóng)業(yè)科技學(xué)院;
【基金】：現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項資金國家牧草產(chǎn)業(yè)技術(shù)體系項目(CARS-35) 中國農(nóng)業(yè)科學(xué)院科技創(chuàng)新工程牧草栽培與加工利用團隊項目(CAAS-ASTIP-IGR 2015-02) 國家自然科學(xué)基金青年科學(xué)基金(31502000) 蘭州大學(xué)中央高�；究蒲袠I(yè)務(wù)費專項資金(lzujbky-2016-8) 國家草品種區(qū)域試驗項目(21301060001) 巴彥淖爾肉羊優(yōu)質(zhì)飼草高效生產(chǎn)關(guān)鍵技術(shù)集成與研究應(yīng)用
【分類號】：S541.9

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