發(fā)布時(shí)間：2018-07-26 12:10

【摘要】：目的:探討薏苡仁不同組分對(duì)脾虛水濕不化模型大鼠空腸extracellular space組件的影響。方法:以高脂低蛋白飲食+游泳建立脾虛水濕不化大鼠模型,基因芯片技術(shù)進(jìn)行空腸全基因組測(cè)定,以Quick-GO注釋到的extracellular space組件進(jìn)行PCA分析。結(jié)果:模型組和正常對(duì)照組具有非常清楚的差異,模型組主要出現(xiàn)了Sepp1、Lamc2、Csn1s1、Mmp8、Ccl20、Apoe、Apoa5、Pyy、Ccl19、Np4基因的上調(diào)和Gnrh1、Col6a3基因的下調(diào),薏苡仁水煎液和各拆分組分均能夠?qū)δＰ痛笫蟮牟町惢蜻M(jìn)行調(diào)節(jié),改善機(jī)體微環(huán)境,主要起作用的基因是Col6a3、Sepp1、Lamc2、Gnrh1、Myoc、Csn1s1、Mmp8、Ccl20、Arg1、Apoe等。結(jié)論:脾虛水濕不化的運(yùn)化失常應(yīng)該是以物質(zhì)由小腸上皮細(xì)胞向其他組織器官運(yùn)送過程的異常為主,薏苡仁各組分的治療都可使失衡的細(xì)胞微環(huán)境得到改善。
[Abstract]:Aim: to investigate the effect of different components of Coix seed on jejunal extracellular space in rats with spleen deficiency and dampness. Methods: the rat model of spleen deficiency without dampness was established by high fat and low protein diet and swimming. The whole genome of jejunum was determined by gene chip technique. The PCA was analyzed by extracellular space module annotated by Quick-GO. Results: there was a very clear difference between the model group and the normal control group. In the model group, the expression of Sepp1OLamc2Csn1s1Csn1s1, Ccl20, Apoa5, Apoa5, and the down-regulation of the Gnrh1 and Col6a3 gene were mainly found in the model group. The Coix seed decoction and the separated components could regulate the differentially expressed genes in the model rats. In order to improve the microenvironment, the major genes that play an important role in improving the microenvironment are Col6a3 Sepp1, Lamc2, Gnrh1, Myocus, Csn1s1, Mmp8, Ccl20, Arg1, Apoe, and so on. Conclusion: the abnormal transport of substances from small intestinal epithelial cells to other tissues and organs should be the main reason for the impotence of spleen deficiency and dampness of water. The treatment of Coix seed components can improve the unbalanced microenvironment of cells.
【作者單位】： 山東中醫(yī)藥大學(xué)中醫(yī)學(xué)院(基礎(chǔ)醫(yī)學(xué)院);山東醫(yī)藥技師學(xué)院;
【基金】：國家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃)資助項(xiàng)目(No.2013CB531803) 山東省中醫(yī)藥科技發(fā)展計(jì)劃項(xiàng)目(No.2015-008)~~
【分類號(hào)】：R285.5

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