發(fā)布時(shí)間：2018-07-23 21:16

【摘要】：該研究克隆了大黃魚(yú)(Larimichthys crocea)gsdf(gonadal soma derived factor)和amh(anti-Müllerian hormone)基因的開(kāi)放閱讀框序列并對(duì)它們的表達(dá)進(jìn)行分析。大黃魚(yú)gsdf基因c DNA序列開(kāi)放閱讀框長(zhǎng)為618 bp,可編碼205個(gè)氨基酸,含有信號(hào)肽和TGF-β結(jié)構(gòu)域。系統(tǒng)進(jìn)化分析顯示,大黃魚(yú)Gsdf與其他魚(yú)類Gsdf聚為一枝,而與TGF-β超家族其他成員分開(kāi)。Amh基因c DNA序列開(kāi)放閱讀框?yàn)? 563 bp,可編碼520個(gè)氨基酸,含有信號(hào)肽、AMH-N區(qū)域和TGF-β保守結(jié)構(gòu)域。系統(tǒng)進(jìn)化分析顯示,大黃魚(yú)Amh與舌齒鱸(Dicentrarchus labrax)Amh進(jìn)化關(guān)系最近。熒光定量PCR表達(dá)分析顯示,gsdf和amh基因主要在大黃魚(yú)性腺表達(dá),在精巢中的表達(dá)量顯著高于卵巢(P0.05),2個(gè)基因都在性腺分化前開(kāi)始表達(dá),在雄魚(yú)精巢中表達(dá)量呈現(xiàn)先升高后下降的趨勢(shì),在卵巢的表達(dá)量很低。此外,相比于正常雌魚(yú),gsdf和amh基因在偽雄魚(yú)(遺傳性雌魚(yú))性腺中的表達(dá)量顯著上調(diào)。這些結(jié)果表明,gsdf和amh基因在大黃魚(yú)性腺分化過(guò)程中起到重要的作用。
[Abstract]:In this study, the open reading frame sequences of (Larimichthys crocea) gsdf (gonadal soma derived factor) and amh (anti-M 眉 llerian hormone) genes of Pseudosciaena Crocea were cloned and analyzed. The open reading frame of c DNA sequence of gsdf gene of large yellow croaker is 618 BP, which encodes 205 amino acids and contains signal peptide and TGF- 尾 domain. Phylogenetic analysis showed that Gsdf of large yellow croaker and other fish Gsdf were one branch, while the c DNA sequence of .Amh gene, which was separated from other members of TGF- 尾 superfamily, was 1 563 BP, encoding 520 amino acids and containing signal peptide AMH-N region and conserved domain of TGF- 尾. Phylogenetic analysis showed that Amh had the closest relationship with (Dicentrarchus labrax) Amh. Fluorescence quantitative PCR analysis showed that GSDF and amh genes were mainly expressed in gonad of Pseudosciaena Crocea, and the expression level in testis was significantly higher than that in ovary (P0.05). Both genes were expressed before gonadal differentiation. In the testis of male fish, the expression level increased first and then decreased, and was very low in ovary. In addition, the expression of GSDF and amh genes in the gonad of pseudomale (hereditary female) was significantly up-regulated than that of normal female. These results suggest that GSDF and amh genes play an important role in gonadal differentiation of Pseudosciaena Crocea.
【作者單位】： 集美大學(xué)水產(chǎn)學(xué)院農(nóng)業(yè)部東海海水健康養(yǎng)殖重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31602207) 廈門南方海洋研究中心重大項(xiàng)目(14GZY70NF34)
【分類號(hào)】：S917.4

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