【摘要】：【目的】植物NPF家族成員具有轉(zhuǎn)運硝酸鹽、小肽等的功能。對水稻OsNPF7.9基因的功能研究能夠為水稻氮素高效利用的分子機制提供理論基礎(chǔ)。【方法】利用不同的生物軟件對OsNPF7.9蛋白的生物學(xué)信息進行了預(yù)測分析;用OsNPF7.9基因啟動子融合GUS報告基因的轉(zhuǎn)基因水稻進行OsNPF7.9的組織定位觀察;利用半定量RT-PCR和p OsNPF7.9::GUS轉(zhuǎn)基因水稻分析OsNPF7.9受氮素調(diào)控特征;構(gòu)建了OsNPF7.9的超表達水稻株系,并進行生理指標(biāo)測定。【結(jié)果】OsNPF7.9是細(xì)胞質(zhì)膜蛋白,有12個跨膜結(jié)構(gòu)域,在第6~7個結(jié)構(gòu)域之間有一個大的親水環(huán)。組織定位結(jié)果顯示OsNPF7.9在根、葉片、根莖結(jié)合處和花中都有表達。RT-PCR和p OsNPF7.9::GUS轉(zhuǎn)基因水稻的GUS染色結(jié)果都表示,OsNPF7.9的表達不受氮素形態(tài)和濃度的影響。OsNPF7.9基因超表達后,不僅顯著增加水稻的地上部硝酸鹽含量以及根系向地上部的硝酸鹽轉(zhuǎn)運,而且增加了地上部的總氮濃度、總氮積累以及根系向地上部的總氮轉(zhuǎn)運。【結(jié)論】OsNPF7.9參與硝酸鹽從根系向地上部的轉(zhuǎn)運,并且OsNPF7.9超表達可以提高水稻氮素積累和轉(zhuǎn)運能力。
[Abstract]:Objective: plant NPF family members have the function of transporting nitrate, small peptides and so on. The functional study of OsNPF7.9 gene in rice could provide a theoretical basis for the molecular mechanism of efficient utilization of nitrogen in rice. [methods] the biological information of OsNPF7.9 protein was predicted and analyzed by different biological software. The tissue localization of OsNPF7.9 transgenic rice fused with Gus reporter gene was observed by using the promoter of OsNPF7.9 gene, and the nitrogen-regulated characteristics of OsNPF7.9 transgenic rice were analyzed by semi-quantitative RT-PCR and p OsNPF7.9: 9: GUS transgenic rice, and the superexpressed rice lines OsNPF7.9 were constructed. [results] OsNPF7.9 is a cytoplasmic membrane protein with 12 transmembrane domains and a large hydrophilic ring between the 6th and 7th domains. The results of tissue localization showed that OsNPF7.9 was expressed in roots, leaves, rhizomes and flowers. RT-PCR and Gus staining of p OsNPF7.9: Gus transgenic rice indicated that the expression of OsNPF7.9 was not affected by nitrogen form and concentration. It not only increased nitrate content in shoot and nitrate transport from root to shoot, but also increased total nitrogen concentration in shoot. [conclusion] OsNPF7.9 is involved in nitrate transport from root to shoot, and OsNPF7.9 overexpression can improve the nitrogen accumulation and transport ability of rice.
【作者單位】： 南京農(nóng)業(yè)大學(xué)資源與環(huán)境科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金資助項目(31401938) 中央高校基本科研業(yè)務(wù)費專項資金資助項目(Y0201500014,Y0201600366)
【分類號】：S511

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1 謝丹;水稻硝酸鹽轉(zhuǎn)運蛋白基因OsNPF7.9表達模式分析及過表達對水稻生長和氮素積累的影響[D];南京農(nóng)業(yè)大學(xué);2014年

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