【摘要】：目的探討KIR3DL3基因測序分型中出現(xiàn)的模棱兩可結(jié)果的鑒定方法。方法對南方漢族614例白血病患者的KIR3DL3基因的全部編碼區(qū)進行測序分型,統(tǒng)計KIR3DL3模棱兩可等位基因結(jié)果的種類及比例,針對每種模棱兩可結(jié)果中等位基因編碼區(qū)序列堿基的差異,設(shè)計組特異性PCR引物,進行組特異性KIR3DL3基因PCR擴增和再測序,鑒定KIR3DL3的基因型。結(jié)果共檢出了7種新的KIR3DL3模棱兩可的結(jié)果,其中3DL3*(00301,01002/00902,028)檢出了7例,占1.14%(7/614);3DL3*(00102,01501/00601,00901/02102,023)、3DL3*(00402,01002/00802,028)、3DL3*(00402,01001/00801,028)分別檢出了6例,均占0.98%(6/614);3DL3*(00301,01001/00901,028)、3DL3*(00301,00801/00402,00901)、3DL3*(00301,00802/00402,00902)分別檢出了3例,均占0.49%(3/614)。這7種模棱兩可組合分別采用組特異性PCR引物均能準確的鑒定出KIR3DL3基因型,其中3DL3*(00102,01501/00601,00901/02102,023)、3DL3*(00402,01001/00801,028)和3DL3*(00301,00802/00402,00902)的模棱兩可組合樣本均鑒定出1種基因型,其余的模棱兩可組合均檢出了2種不同的基因型。結(jié)論本文為鑒定KIR3DL3模棱兩可的結(jié)果建立了可靠的鑒定方法,具有良好的實用價值。
[Abstract]:Objective to investigate the identification of ambiguous results in KIR3 DL3 genotyping. Methods all coding regions of KIR3DL3 gene in 614 patients with leukemia in southern Han nationality were sequenced, and the types and proportions of the results of KIR3DL3 ambiguous alleles were analyzed. The gene of KIR3DL3 gene was amplified by PCR and sequenced. The genotypes of KIR3DL3 gene were identified. 緇撴灉鍏辨鍑轟簡7縐嶆柊鐨凨IR3DL3妯℃１涓ゅ彲鐨勭粨鏋，

本文編號：2126630