2014-2016年柳州市甲型H3流感病毒血凝素抗原表位基因變異和進(jìn)化分析
發(fā)布時(shí)間：2018-07-15 22:01
【摘要】：目的通過分析近年柳州市甲型H3流感病毒HA抗原表位基因突變情況和進(jìn)化分析,了解其分子流行及變異變遷規(guī)律。方法采集2014-2016年柳州市哨點(diǎn)醫(yī)院以及流感突發(fā)疫情病例的呼吸道標(biāo)本。提取標(biāo)本核酸后用實(shí)時(shí)熒光PCR檢測(cè),對(duì)甲型H3陽性標(biāo)本擴(kuò)增血凝素HA基因片段、測(cè)序和進(jìn)行基因變異分析。結(jié)果在2014-2016年間總共檢測(cè)5 164例病例,503例為甲型H3流感病毒陽性。分析2014-2016年柳州市獲得的41個(gè)甲型H3流感病毒HA基因全長(zhǎng)序列的系統(tǒng)進(jìn)化樹發(fā)現(xiàn):2014-2016年間甲型H3流感病毒共引起了3個(gè)主要的流感流行峰,且分別屬于兩個(gè)不同的基因簇A/Switzerland/9715293/2013類似株(SW13)為Group1和A/Hong Kong/4801/2014類似株(HK14)Group 2。比較3個(gè)流行峰發(fā)現(xiàn)血凝素蛋白氨基酸序列出現(xiàn)點(diǎn)突變,主要分布在抗原表位和受體結(jié)合位點(diǎn)。結(jié)論2014-2016年柳州市流行的甲型H3流感病毒,與當(dāng)年WHO推薦疫苗株比較出現(xiàn)滯后現(xiàn)象,出現(xiàn)兩次明顯的抗原漂變,可能是導(dǎo)致柳州市近年來甲型H3流感流行的原因。
[Abstract]:Objective to analyze the mutation and evolution of HA epitope gene of influenza A (H3) virus in Liuzhou City in recent years and to understand its molecular prevalence and variation. Methods Respiratory tract specimens from sentinel hospital and influenza outbreak cases were collected from 2014-2016. The HA gene fragment of hemagglutinin was amplified and sequenced and analyzed by real-time fluorescence PCR. Results A total of 5 164 cases were detected in 2014-2016. 503 cases were positive for influenza A H 3 virus. The phylogenetic tree of 41 full-length HA gene sequences of influenza A / H3 virus obtained from Liuzhou City from 2014-2016 shows that the influenza A / H3 virus caused three major influenza epidemic peaks between 2014 and 2016. And belong to two different clusters of gene clusters, A / R / 9715293 / 2013 (SW13), respectively, are Group1 and A / Hong Kong.4801 / 2014 (HK14) Group 2. The amino acid sequence of hemagglutinin protein was found to have point mutations, mainly in antigen epitopes and receptor binding sites. Conclusion compared with the vaccine strains recommended by WHO, the influenza A H3 virus in Liuzhou from 2014-2016 to 2016 is lagging behind, and two obvious antigen bleaching appears, which may be the cause of influenza A H3 epidemic in Liuzhou in recent years.
【作者單位】： 柳州市疾病預(yù)防控制中心微生物檢驗(yàn)科;
【基金】：廣西衛(wèi)生廳自籌課題(E2011265)
【分類號(hào)】：R511.7
，
本文編號(hào)：2125511
[Abstract]:Objective to analyze the mutation and evolution of HA epitope gene of influenza A (H3) virus in Liuzhou City in recent years and to understand its molecular prevalence and variation. Methods Respiratory tract specimens from sentinel hospital and influenza outbreak cases were collected from 2014-2016. The HA gene fragment of hemagglutinin was amplified and sequenced and analyzed by real-time fluorescence PCR. Results A total of 5 164 cases were detected in 2014-2016. 503 cases were positive for influenza A H 3 virus. The phylogenetic tree of 41 full-length HA gene sequences of influenza A / H3 virus obtained from Liuzhou City from 2014-2016 shows that the influenza A / H3 virus caused three major influenza epidemic peaks between 2014 and 2016. And belong to two different clusters of gene clusters, A / R / 9715293 / 2013 (SW13), respectively, are Group1 and A / Hong Kong.4801 / 2014 (HK14) Group 2. The amino acid sequence of hemagglutinin protein was found to have point mutations, mainly in antigen epitopes and receptor binding sites. Conclusion compared with the vaccine strains recommended by WHO, the influenza A H3 virus in Liuzhou from 2014-2016 to 2016 is lagging behind, and two obvious antigen bleaching appears, which may be the cause of influenza A H3 epidemic in Liuzhou in recent years.
【作者單位】： 柳州市疾病預(yù)防控制中心微生物檢驗(yàn)科;
【基金】：廣西衛(wèi)生廳自籌課題(E2011265)
【分類號(hào)】：R511.7
，
本文編號(hào)：2125511
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